FastQCFastQC Report
Wed 28 Aug 2019
H2HY7BGXC_n02_S22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HY7BGXC_n02_S22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7327925
Sequences flagged as poor quality0
Sequence length25
%GC4

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT265619736.24760078739889No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA5435747.41784338677047No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA2333243.184039137955151No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT1448931.977271874370985No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG1229931.6784151038663744No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC1223701.669913379299051No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT1194801.6304752027347442No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA982651.3409662353258256No Hit
TTTTTTTTTTTTTTTTTTTTTTGTT620010.8460921747970948No Hit
TTTTTTTTTTTTTTTTTTTTTTATT601560.820914515364172No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC536020.7314758270588195No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC433490.5915590020367294No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT343880.4692733618316235No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA317440.4331922065250395No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA317100.4327282279772241No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA271970.3711418989686712No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC269950.36838532053753276No Hit
TTTTTTTTTTTTTTTTTTTTTTTCT267760.3653967528324867No Hit
TTTTTTTTTTTTTTTTTTTTTATTT225550.30779518076399526No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC210060.28665686398264173No Hit
TTTTTTTTTTTTTTTTTTTTTTACA207250.2828222177492264No Hit
TTTTTTTTTTTTTTTTTTTTTTACC188330.25700317620608837No Hit
TTTTTTTTTTTTTTTTTTTTTAATT182110.24851509806664232No Hit
TTTTTTTTTTTTTTTTTTTTTTCTT176290.24057287704227323No Hit
TTTTTTTTTTTTTTTTTTTTTTCAA160840.21948914597242739No Hit
TTTTTTTTTTTTTTTTTTTTTGTTT151690.20700266446504298No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG147830.20173514330455075No Hit
TGTTTTTTTTTTTTTTTTTTTTTTT145970.1991969077194431No Hit
TTTTTATTTTTTTTTTTTTTTTTTT141410.19297413660756627No Hit
TTTTTTTTTTTTTTTTTTTTTTATA138130.18849810826393557No Hit
TTTTTTTTATTTTTTTTTTTTTTTT126080.17205416267224352No Hit
TTTATTTTTTTTTTTTTTTTTTTTT124120.16937946280836663No Hit
TTTTTTATTTTTTTTTTTTTTTTTT123400.16839692000122816No Hit
TTTTTTTTTTTTTTTTTTTTTTCCC117020.15969049901575139No Hit
TTGTTTTTTTTTTTTTTTTTTTTTT111020.15150264228959767No Hit
TTATTTTTTTTTTTTTTTTTTTTTT110210.15039728163156693No Hit
TTTTTTTTTTTTTTTTTTTTTTCCA105250.14362865340461317No Hit
TTTTTTTTTTTTTTTTTTTTTACAA102290.139589310753044No Hit
TTTTATTTTTTTTTTTTTTTTTTTT101250.13817008225384403No Hit
TTTTTTTATTTTTTTTTTTTTTTTT99010.1351132824094133No Hit
TTTGTTTTTTTTTTTTTTTTTTTTT97000.1323703504061518No Hit
TTTTTTTTTTTTTTTGTTTTTTTTT94850.1294363684126134No Hit
TTTTTTTTTTTTTTTTTTTTTAAAT92010.1255607828955673No Hit
TTTTGTTTTTTTTTTTTTTTTTTTT91050.1242507258193827No Hit
TTTTTTTTTTTTTTTTTTTTTTGGT90510.12351381871402888No Hit
TTTTTGTTTTTTTTTTTTTTTTTTT89750.12247669019538274No Hit
TTTTTTTTTTTTTATTTTTTTTTTT88290.120484311725352No Hit
TTTTTTGTTTTTTTTTTTTTTTTTT82830.1130333621045521No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT82030.11194164787439828No Hit
TTTTTTTTTTTTTTTTTTTTTCTTT80860.1103450158127983No Hit
TTTTTTTTTTTTTTTTTTTTTACCA80460.10979915869772137No Hit
TTTTTTTTTTTTTTTTTTTTTTTAG80090.10929424086627525No Hit
TTTTTTTTTTTTTGTTTTTTTTTTT79030.10784771951132142No Hit
TTTTTTTTTTTTTTTTTTTTATTTT78910.10768396237679835No Hit
TTTTTTTTTTATTTTTTTTTTTTTT78830.10757479095378296No Hit
TTTTTTTTTTTATTTTTTTTTTTTT78720.1074246802471368No Hit
TTTTTTTTTTTTTTTTATTTTTTTT78590.1072472766847368No Hit
TTTTTTTTTTTTGTTTTTTTTTTTT78520.10715175168959837No Hit
TTTTTTTTGTTTTTTTTTTTTTTTT77530.10580075532978298No Hit
TTTTTTTTTTTTTTTTTTTTTTACT76990.10506384822442916No Hit
TTTTTTTTTTTTTTTTGTTTTTTTT76750.10473633395538301No Hit
TTTTTTTTTTTTTTTTTTTTTTCCT74230.10129743413039845No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCTA359.854462E-519.0229171
TGGGCTA359.854462E-519.0229171
TGGCCTA552.8045179E-819.0229171
GGCCTAA552.8267095E-819.0097982
GGGCTAA307.6997274E-419.0097982
CGCTAAA250.006028576319.0030523
GGCTAAA359.9338E-519.0030523
CCCTAAC359.9338E-519.0030523
CCTAACC250.006035947218.9990314
GCTAAAC401.2868404E-518.9990314
GCTAAAA307.729122E-418.9990314
CGTAAAA250.006035947218.9990314
CTAACCC250.00603785118.9979935
CTAAAAC1050.018.9979935
GGTAAGG552.8469003E-818.99799315
TTAAGGT502.1785854E-718.99786616
GTAAGGT603.7307473E-918.99786616
TGGTAAG850.018.99786414
CACCGCC401.287522E-518.99786413
CCACCTC401.287522E-518.99786412
Produced by FastQC (version 0.11.5)