FastQCFastQC Report
Wed 28 Aug 2019
H2HY7BGXC_n02_S21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HY7BGXC_n02_S21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7097874
Sequences flagged as poor quality0
Sequence length25
%GC4

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT257935236.339782870194654No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA4778746.732635715990449No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA2000732.8187736215097647No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT1511412.129384094448563No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG1225911.7271509750666185No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC1086791.5311486228129718No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT1068841.505859360140797No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA838531.1813819180222134No Hit
TTTTTTTTTTTTTTTTTTTTTTGTT662370.9331949256918339No Hit
TTTTTTTTTTTTTTTTTTTTTTATT569160.8018739132309195No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC477050.6721026605994977No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC387500.545938121753077No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA314300.4428086494632054No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT289100.4073050606421021No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA274420.38662281128123716No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC252130.35521904164542795No Hit
TTTTTTTTTTTTTTTTTTTTTTTCT240370.3386507001955797No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA228360.32173014060266497No Hit
TTTTTTTTTTTTTTTTTTTTTATTT215060.3029921353915271No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC184610.2600919655660272No Hit
TTTTTTTTTTTTTTTTTTTTTGTTT183800.2589507787824918No Hit
TTTTTTTTTTTTTTTTTTTTTTACA176950.24930000166246963No Hit
TTTTTTTTTTTTTTTTTTTTTTACC170460.24015641866846327No Hit
TTTTTTTTTTTTTTTTTTTTTTCTT158750.22365852084722834No Hit
TTTTTTTTTTTTTTTTTTTTTAATT155030.2184175148783988No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG153000.2155575035566988No Hit
TGTTTTTTTTTTTTTTTTTTTTTTT146780.20679431615720426No Hit
TTTTTTTTTTTTTTTTTTTTTTCAA139670.19677723216839296No Hit
TTTTTATTTTTTTTTTTTTTTTTTT139570.19663634491116638No Hit
TTTTTTTTTTTTTTTTTTTTTTATA126940.1788422843234467No Hit
TTTTTTTTATTTTTTTTTTTTTTTT124980.17608089408180536No Hit
TTGTTTTTTTTTTTTTTTTTTTTTT123020.17331950384016395No Hit
TTTATTTTTTTTTTTTTTTTTTTTT122750.17293910824565215No Hit
TTTTTTATTTTTTTTTTTTTTTTTT121630.1713611709647142No Hit
TTTTTTTTTTTTTTTGTTTTTTTTT117820.16599336646438073No Hit
TTTTTTTTTTTTTTTTTTTTTTCCC113430.159808415872133No Hit
TTTGTTTTTTTTTTTTTTTTTTTTT106550.15011537257494287No Hit
TTTTTTTTTTTTTTTTTTTTTTGGT104660.1474526034133601No Hit
TTATTTTTTTTTTTTTTTTTTTTTT104220.14683269948156308No Hit
TTTTTGTTTTTTTTTTTTTTTTTTT103450.14574786760091826No Hit
TTTTTTTTTTTTTTTTGTTTTTTTT101780.14339505040523404No Hit
TTTTGTTTTTTTTTTTTTTTTTTTT100940.14221159744453057No Hit
TTTTTTTATTTTTTTTTTTTTTTTT100180.1411408542896084No Hit
TTTTATTTTTTTTTTTTTTTTTTTT98240.13840764149941237No Hit
TTTTTTTTTTTTTGTTTTTTTTTTT96800.1363788649953493No Hit
TTTTTTTTTTTTTTTTTTTTGTTTT96340.13573078361210694No Hit
TTTTTTTTTTTTGTTTTTTTTTTTT95480.1345191531999582No Hit
TTTTTTGTTTTTTTTTTTTTTTTTT95290.13425146741122765No Hit
TTTTTTTTTTTTTTTTTTTTTTCCA94400.13299757082191088No Hit
TTTTTTTTGTTTTTTTTTTTTTTTT89150.12560098981751436No Hit
TTTTTTTTTTTTTATTTTTTTTTTT88340.1244598030339789No Hit
TTTTTTTTTTTTTTTTTTTTTACAA87000.12257191378714247No Hit
TTTTTTTTTTTTTTGTTTTTTTTTT82620.11640105192061735No Hit
TTTTTTTTTTTTTTTTTGTTTTTTT82380.1160629225032735No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT81570.11492173571973806No Hit
TTTTTTTTTTTTTTTTTTTTATTTT80140.11290704794139766No Hit
TTTTTTTTTTTTTTTTTTTTTCTTT79490.11199128076942477No Hit
TTTTTTTTTTTTTTTTATTTTTTTT77770.10956801994512723No Hit
TTTTTTTTTTTGTTTTTTTTTTTTT77260.10884949493327158No Hit
TTTTTTTTTTTTTTTTTTTTTAAAT77040.10853954296737305No Hit
TTTTTTTTTTTATTTTTTTTTTTTT76900.1083423008072558No Hit
TTTTTTTTTTATTTTTTTTTTTTTT75410.10624308067457945No Hit
TTTTTTTTTTTTTTTTTTGTTTTTT74380.10479194192514547No Hit
TTTTTTTTTTTTTTTTTTTTTTTAG74050.1043270139762977No Hit
TTTTTTTTTTGTTTTTTTTTTTTTT72950.1027772541468051No Hit
TTTTTTTTTTTTATTTTTTTTTTTT71620.10090345362569131No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGCTA359.8736564E-519.0180821
TGGCCTA502.1543201E-719.0180821
GGCCTAA401.2820561E-519.0072252
GGCTAAA307.7195035E-419.0025393
GCCTAAA502.1729284E-719.0025373
CCTAAAC1000.018.9987894
GTAAGTT654.8930815E-1018.99838816
GTGAGGT307.7308505E-418.99838616
GTAAGGT552.8463546E-818.99838616
TAAGGTT850.018.99838617
GGTGAGG250.00603735718.99825515
TTGTAAG654.8930815E-1018.99825314
TGGTAAG654.8930815E-1018.99825314
TGTAAGT451.6715876E-618.99825315
GGTAAGG451.6715876E-618.99825315
CACCGCC451.6716986E-618.9981213
GTGGTGA250.006037602718.9981213
AGGTTGC307.7319494E-418.99798619
AGTTTGC307.7319494E-418.99798619
AGTTTCC307.7319494E-418.99798619
Produced by FastQC (version 0.11.5)