FastQCFastQC Report
Wed 28 Aug 2019
H2HY7BGXC_n02_S20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HY7BGXC_n02_S20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8296923
Sequences flagged as poor quality0
Sequence length25
%GC5

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT306731236.96927162033443No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA4395155.297325285530551No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT2047832.468180071093826No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA1910192.302287245524636No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG1392951.678875409594617No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT1162281.4008566790363126No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC1011711.2193797628349692No Hit
TTTTTTTTTTTTTTTTTTTTTTGTT1008381.2153662267324887No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA822790.9916808918197746No Hit
TTTTTTTTTTTTTTTTTTTTTTATT698890.8423484224211795No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC402600.48524013058817106No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC350880.4229037680595566No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT315030.3796949784878081No Hit
TTTTTTTTTTTTTTTTTTTTTGTTT298160.35936213943410106No Hit
TTTTTTTTTTTTTTTTTTTTTATTT292670.35274522856244417No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA270150.3256026360615857No Hit
TTTTTTTTTTTTTTTTTTTTTTTCT255760.3082588569280443No Hit
TGTTTTTTTTTTTTTTTTTTTTTTT236140.28461153610802464No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA234740.28292416357244726No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA230570.27789820394862047No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC209760.25281661647336007No Hit
TTTTTTTTTTTTTTTGTTTTTTTTT209350.2523224573736553No Hit
TTGTTTTTTTTTTTTTTTTTTTTTT199250.24014926979556156No Hit
TTTTTGTTTTTTTTTTTTTTTTTTT190100.22912108500946676No Hit
TTTTTTTTTTTTTTTTTTTTTTCTT188900.2276747656932576No Hit
TTTGTTTTTTTTTTTTTTTTTTTTT188010.22660207886706912No Hit
TTTTTTTTTTTTTTTTTTTTTAATT180270.2172733192775201No Hit
TTTTGTTTTTTTTTTTTTTTTTTTT180250.21724921395558328No Hit
TTTTTTGTTTTTTTTTTTTTTTTTT175690.2117532005539885No Hit
TTTTTTTTTTTTTTTTGTTTTTTTT170960.20605229191593077No Hit
TTTTTTTTTTTTTTTTTTTTTTACA170240.20518450032620525No Hit
TTTTTTTTTTTTGTTTTTTTTTTTT169490.20428055075357457No Hit
TTTTTTTTTTTTTGTTTTTTTTTTT169090.20379844431483818No Hit
TTTTTTTTTTTTTTTTTTTTGTTTT163420.19696458554574992No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC161100.19416836820107888No Hit
TTTTTTTTGTTTTTTTTTTTTTTTT160750.19374652506718454No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG153390.18487576659443508No Hit
TTTTTTTTTTTTTTTTTTTTTTACC144140.17372705519865617No Hit
TTTTTTTTTTTTTTGTTTTTTTTTT142520.17177452412177383No Hit
TTTTTTTTTTTTTTTTTTTTTTCAA139000.16753198746089362No Hit
TTTTTTTTTTTGTTTTTTTTTTTTT138630.16708603900506247No Hit
TTTTTTTTTTTTTTTTTGTTTTTTT138460.16688114376859953No Hit
TTTTTTTTTTTTTTTTTTTTTTGGT136210.16416929505070735No Hit
TTTTTTTTTTGTTTTTTTTTTTTTT129900.15656406597964087No Hit
TTTTTATTTTTTTTTTTTTTTTTTT129580.15617838082865176No Hit
TTTTTTTTTTTTTTTTTTGTTTTTT125790.15161042232162453No Hit
TTTTTTTTATTTTTTTTTTTTTTTT122310.147416096304618No Hit
TTTTTTATTTTTTTTTTTTTTTTTT118130.14237808401982277No Hit
TTTTTTTGTTTTTTTTTTTTTTTTT117230.1412933445326659No Hit
TTTATTTTTTTTTTTTTTTTTTTTT114700.13824402130765828No Hit
TTTTTTTTTGTTTTTTTTTTTTTTT114500.1380029680882901No Hit
TTTTTTTTTTTTTTTTTTTGTTTTT110830.13357964151288376No Hit
TTTTTTTTTTTTTTTTTTTTTTATA108370.130614686914655No Hit
TTATTTTTTTTTTTTTTTTTTTTTT99800.12028555646472795No Hit
TTTTTTTTTTTTTTTTTTTTTCTTT99570.12000834526245453No Hit
TTTTTTTATTTTTTTTTTTTTTTTT97950.11805581418557218No Hit
TTTTTTTTTTTTTTTTTTTTATTTT95840.11551270272123774No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT94690.11412664670987065No Hit
TTTTATTTTTTTTTTTTTTTTTTTT94330.11369275091500788No Hit
TTTTTTTTTTTTTTTTTTTTTTCCC91690.11051084841934775No Hit
TTTTTTTTTTTTTATTTTTTTTTTT89890.10834136944503402No Hit
TTTTTTTTTTTTTTTTTTTTTACAA87430.10537641484680525No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGCTA250.005999668519.0189081
TGGCCTA451.65436E-619.0189081
GGCCTAA451.6645154E-619.0067522
GCCTAAA552.8410795E-819.0014823
GGCTAAA250.00603151719.0014823
AGTGGGG401.2870472E-518.99873411
CCACCTC359.9513396E-518.99873412
TAAGATT307.730366E-418.99862117
TAAGGTT1700.018.9986217
GTAAGTT850.018.99850716
GTGAGGT654.8930815E-1018.99850516
GTAAGGT1450.018.99850516
TAGTGGG359.9527206E-518.99839210
GGTGAGG654.8930815E-1018.99827815
GGTAAGT401.2873146E-518.99827815
GTTAAGG307.731306E-418.99827615
CTAAACC706.366463E-1118.9981615
CACCGCC307.7319326E-418.99804713
CACCTCC359.9541016E-518.99804713
GTGGTGA603.7307473E-918.99804713
Produced by FastQC (version 0.11.5)