FastQCFastQC Report
Wed 28 Aug 2019
H2HY7BGXC_n02_S1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HY7BGXC_n02_S1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8389636
Sequences flagged as poor quality0
Sequence length25
%GC6

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT292595834.875863505877966No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA5134866.120480078039143No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA2141532.552589885902082No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT1972472.351079355528655No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG1516361.8074204888030898No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT1311801.56359584611299No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC1284251.5307577110616004No Hit
TTTTTTTTTTTTTTTTTTTTTTGTT916471.092383507460872No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA877801.0462909237063442No Hit
TTTTTTTTTTTTTTTTTTTTTTATT726840.8663546308802907No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC528150.6295267160577646No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC461360.5499165875611289No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT360990.43028088465339853No Hit
TTTTTTTTTTTTTTTTTTTTTTTCT308070.3672030586309108No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA270530.32245737478956177No Hit
TTTTTTTTTTTTTTTTTTTTTATTT270200.32206403233704056No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA269530.32126542796374No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA258670.30832088543531566No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC251380.29963159307507503No Hit
TTTTTTTTTTTTTTTTTTTTTGTTT250470.2985469214635772No Hit
TTTTTTTTTTTTTTTTTTTTTTCTT206560.24620853634174356No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC206380.24599398591309565No Hit
TTTTTTTTTTTTTTTTTTTTTAATT190600.2271850650016282No Hit
TTTTTTTTTTTTTTTTTTTTTTACC188500.22468197666740247No Hit
TTTTTTTTTTTTTTTTTTTTTTACA183860.2191513433955895No Hit
TGTTTTTTTTTTTTTTTTTTTTTTT174730.20826886887583682No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG166210.1981134819198354No Hit
TTTTTTTTTTTTTTTGTTTTTTTTT156890.18700453750317653No Hit
TTGTTTTTTTTTTTTTTTTTTTTTT144170.1718429738787237No Hit
TTTTTTTTTTTTTTTTTTTTTTCAA143890.1715092287674936No Hit
TTTTTGTTTTTTTTTTTTTTTTTTT138990.16566868932096698No Hit
TTTTTATTTTTTTTTTTTTTTTTTT137690.16411915844739866No Hit
TTTTTTTTTTTTTTTTTTTTGTTTT131810.1571105111115667No Hit
TTTTTTTTTTTTTTTTGTTTTTTTT130930.15606159790484353No Hit
TTTTTTTTTTTTTTTTTTTTTTATA128520.1531890060546131No Hit
TTTTTTTTTTTTTGTTTTTTTTTTT127920.15247383795912003No Hit
TTTTTTGTTTTTTTTTTTTTTTTTT127460.15192554241924203No Hit
TTTGTTTTTTTTTTTTTTTTTTTTT127150.15155603890323727No Hit
TTTTTTTTTTTTTTTTTTTTTTGGT126920.1512818911332983No Hit
TTTTTTTTTTTTGTTTTTTTTTTTT124500.1483973798148096No Hit
TTTTGTTTTTTTTTTTTTTTTTTTT121530.14485729774211897No Hit
TTTTTTATTTTTTTTTTTTTTTTTT120340.14343888101939106No Hit
TTTATTTTTTTTTTTTTTTTTTTTT119540.14248532355873367No Hit
TTTTTTTTGTTTTTTTTTTTTTTTT118210.14090003428039072No Hit
TTTTTTTTATTTTTTTTTTTTTTTT117580.140149107780123No Hit
TTTTTTTTTTTTTTTTTTTTTTCCC116060.13833734860487393No Hit
TTTTTTTTTTTTTTGTTTTTTTTTT108330.12912359964127168No Hit
TTTTTTTTTTTTTTTTTGTTTTTTT108290.12907592176823882No Hit
TTATTTTTTTTTTTTTTTTTTTTTT106450.12688273960872679No Hit
TTTTTTTTTTTTTTTTTTTTTTCCT101780.12131634793213913No Hit
TTTTTTTTTTTGTTTTTTTTTTTTT100870.12023167632064131No Hit
TTTTTTTTTTTTTTTTTTTTTTTAG100410.11968338078076331No Hit
TTTTTTTATTTTTTTTTTTTTTTTT97700.11645320488278635No Hit
TTTTTTTTTTTTTTTTTTTTTTACT97630.11636976860497882No Hit
TTTTTTTTTTTTTTTTTTGTTTTTT97310.11598834562071585No Hit
TTTTTTTTTTGTTTTTTTTTTTTTT97040.11566651997774396No Hit
TTTTATTTTTTTTTTTTTTTTTTTT96710.11527317752522279No Hit
TTTTTTTTTTTTTTTTTTTTTCTTT93900.11192380694466363No Hit
TTTTTTTTTTTTTTTTTTTTATTTT92400.11013588670593097No Hit
TTTTTTTTTTTTTTTTTTTTTAAAT92340.11006436989638169No Hit
TTTTTTTTTTTTTTTTTTTTTTCCA91300.10882474519752705No Hit
TTTTTTTTTTTTTTTTTTTGTTTTT91280.10880090626101062No Hit
TTTTTTTTTTTTTATTTTTTTTTTT88950.1060236701568459No Hit
TTTTTTTGTTTTTTTTTTTTTTTTT86860.10353250129087842No Hit
TTTTTTTTTTTTTTTTTTTTTTAGT86330.10290076947319289No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT86250.10280541372712715No Hit
TTTTTTTTTTTTTTTTTTTTTACAA86160.10269813851280318No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTA307.671259E-419.0203191
TCCGCTA250.005997107419.0203191
TGGGCTA654.820322E-1019.0203171
GGCGTAA307.7082776E-419.006712
GCCGTAA307.7082776E-419.006712
GCCCAAA359.919361E-519.0067082
CCGCTAA250.006021952319.0067082
GGCCTAA1350.019.0067082
GGCCAAA250.006021952319.0067082
GGGCTAA552.8321665E-819.0067082
GGGTATA307.723127E-419.001273
GCCTAAA1650.019.001273
CGCTAAA552.8414433E-819.001273
CCGTAAA401.2855702E-519.001273
GGCTAAA603.7234713E-919.001273
GCGTAAA401.2855702E-519.001273
CCCTAAC250.00603191419.0012683
CCTCAAC359.951179E-518.998784
GGTGATG250.00603648418.9987774
GCTAAAC603.7289283E-918.9987774
Produced by FastQC (version 0.11.5)