FastQCFastQC Report
Wed 28 Aug 2019
H2HY7BGXC_n02_S19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HY7BGXC_n02_S19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8132358
Sequences flagged as poor quality0
Sequence length25
%GC6

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT289493935.59778111096437No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA4077095.013416772847433No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT2043412.5126906611833864No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA1763452.1684362641192134No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG1409431.7331135692747417No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT1090391.3408042292284723No Hit
TTTTTTTTTTTTTTTTTTTTTTGTT1025001.2603970459736278No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC943831.1605858964890625No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA746430.917851870269361No Hit
TTTTTTTTTTTTTTTTTTTTTTATT654770.8051416329679534No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC361120.4440532499921917No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC322750.39687136252486677No Hit
TTTTTTTTTTTTTTTTTTTTTGTTT302960.37253647712016613No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT292640.35984643076460726No Hit
TTTTTTTTTTTTTTTTTTTTTATTT269200.33102330222058596No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA245720.3021509874503803No Hit
TTTTTTTTTTTTTTTTTTTTTTTCT238940.29381392211213525No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA220940.27168012032918376No Hit
TGTTTTTTTTTTTTTTTTTTTTTTT211040.2595065293485604No Hit
TTTTTTTTTTTTTTTGTTTTTTTTT210680.2590638533129014No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA208820.2567766937953297No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC191850.2359094373366249No Hit
TTGTTTTTTTTTTTTTTTTTTTTTT185080.2275846685549259No Hit
TTTTTGTTTTTTTTTTTTTTTTTTT178710.21975176203507027No Hit
TTTTTTTTTTTTTTTTTTTTTTCTT178220.21914923076431217No Hit
TTTTTTTTTTTTTGTTTTTTTTTTT173520.2133698491876526No Hit
TTTGTTTTTTTTTTTTTTTTTTTTT173210.21298865593472396No Hit
TTTTTTTTTTTTTTTTGTTTTTTTT171100.2103940825035002No Hit
TTTTTTTTTTTTGTTTTTTTTTTTT167700.20621325327783158No Hit
TTTTTTGTTTTTTTTTTTTTTTTTT166260.20444254913519547No Hit
TTTTTTTTTTTTTTTTTTTTGTTTT165820.20390150064716778No Hit
TTTTTTTTTTTTTTTTTTTTTAATT164160.20186027226051778No Hit
TTTTGTTTTTTTTTTTTTTTTTTTT163190.20066750627554764No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG161150.19815900874014647No Hit
TTTTTTTTGTTTTTTTTTTTTTTTT152390.18738722520577672No Hit
TTTTTTTTTTTTTTTTTTTTTTACA151710.186551059360643No Hit
TTTTTTTTTTTTTTTTTGTTTTTTT150610.18519843814057374No Hit
TTTTTTTTTTTTTTGTTTTTTTTTT144260.1773901247338103No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC142630.17538578601679858No Hit
TTTTTATTTTTTTTTTTTTTTTTTT140530.1728035091421209No Hit
TTTTTTTTTTTGTTTTTTTTTTTTT137870.1695326251008625No Hit
TTTTTTTTTTTTTTTTTTTTTTGGT135110.16613877549414327No Hit
TTTTTTTTTTTTTTTTTTTTTTACC131600.16182268414646772No Hit
TTTTTTTTTTTTTTTTTTGTTTTTT128830.1584165379832024No Hit
TTTTTTTTTTGTTTTTTTTTTTTTT128220.15766644803389127No Hit
TTTTTTTTTTTTTTTTTTTTTTCAA127000.15616626813526902No Hit
TTTTTTTTATTTTTTTTTTTTTTTT122880.15110008683828233No Hit
TTTTTTATTTTTTTTTTTTTTTTTT120100.14768164411847093No Hit
TTTATTTTTTTTTTTTTTTTTTTTT119220.14659954714241552No Hit
TTTTTTTTTTTTTTTTTTTGTTTTT117950.14503788446106283No Hit
TTTTTTTGTTTTTTTTTTTTTTTTT116720.14352540800589447No Hit
TTTTTTTTTGTTTTTTTTTTTTTTT106350.1307738788676052No Hit
TTTTTTTTTTTTTTTTTTTTTTATA101110.12433048323745707No Hit
TTATTTTTTTTTTTTTTTTTTTTTT100530.1236172829577842No Hit
TTTTTTTATTTTTTTTTTTTTTTTT96390.11852650854770536No Hit
TTTTATTTTTTTTTTTTTTTTTTTT94570.11628853525631802No Hit
TTTTTTTTTTTTTTTTTTTTTCTTT91710.11277172008413797No Hit
TTTTTTTTTTTTTTTTTTTTATTTT91000.11189866456936598No Hit
TTTTTTTTTTTTTATTTTTTTTTTT89030.10947624292978739No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT84730.1041887236149712No Hit
TTTTTTTTTTTTTTTTTTTTTTCCC83660.1028729920645402No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCTA654.820322E-1019.0188871
TCGCCTC250.005999703419.0188851
TGGGCTA1000.019.0188851
TCCGCTA451.6543727E-619.0188851
TGGCCTA1850.019.0188851
TCCCGTA552.81143E-819.0188831
TCCCCGA359.870684E-519.0188831
GGCGTTA250.006021706419.0068342
CGCCTAA603.7089194E-919.0068342
CCCGTAA603.7089194E-919.0068342
GGCCTCA250.006021706419.0068342
GGCCTAA1750.019.0068342
ACCCTAA250.006021706419.0068342
GGCCAAA250.006021706419.0068342
GGGCTGA401.2823208E-519.0068342
GGGCTCA250.006021706419.0068342
GCCTCAA307.7213114E-419.0019253
GCCTAAA2400.019.0019253
GGCTGAA359.938494E-519.0019253
GGCTCAA250.006030697419.0019253
Produced by FastQC (version 0.11.5)