FastQCFastQC Report
Wed 28 Aug 2019
H2HY7BGXC_n02_S18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HY7BGXC_n02_S18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7665723
Sequences flagged as poor quality0
Sequence length25
%GC5

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT281401336.70903579479718No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA4411915.755373628814921No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA1882852.45619362974634No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT1834152.392664070955864No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG1298191.6934997520781796No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT1125861.4686938205306923No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC1008121.315101002214664No Hit
TTTTTTTTTTTTTTTTTTTTTTGTT865661.1292607363975975No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA796631.039210521955985No Hit
TTTTTTTTTTTTTTTTTTTTTTATT659530.8603624211310531No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC418500.5459367629119914No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC357870.46684441898044055No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT309650.4039410242191115No Hit
TTTTTTTTTTTTTTTTTTTTTATTT265930.3469079172310296No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA261380.34097240403807966No Hit
TTTTTTTTTTTTTTTTTTTTTTTCT255060.3327279109876524No Hit
TTTTTTTTTTTTTTTTTTTTTGTTT250810.32718375031291896No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA242430.31625196997073857No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA230750.30101531192817693No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC213630.27868212822195637No Hit
TGTTTTTTTTTTTTTTTTTTTTTTT192310.2508700092607051No Hit
TTTTTTTTTTTTTTTTTTTTTTCTT183140.23890766728722132No Hit
TTTTTTTTTTTTTTTTTTTTTAATT177390.2314067440214054No Hit
TTTTTTTTTTTTTTTTTTTTTTACA169340.22090545144926318No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC164530.21463076607385895No Hit
TTGTTTTTTTTTTTTTTTTTTTTTT163340.21307840108493353No Hit
TTTTTTTTTTTTTTTGTTTTTTTTT162670.21220438046091672No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG152580.1990418907649024No Hit
TTTTTTTTTTTTTTTTTTTTTTACC150820.1967459559913657No Hit
TTTGTTTTTTTTTTTTTTTTTTTTT149100.19450220155359121No Hit
TTTTTGTTTTTTTTTTTTTTTTTTT146840.19155401258302707No Hit
TTTTGTTTTTTTTTTTTTTTTTTTT141010.1839487286456868No Hit
TTTTTTTTTTTTTTTTTTTTTTCAA137740.17968298619712714No Hit
TTTTTTTTTTTTTTTTGTTTTTTTT136390.17792189986515297No Hit
TTTTTTGTTTTTTTTTTTTTTTTTT136070.17750445717905539No Hit
TTTTTTTTTTTTTGTTTTTTTTTTT134010.17481716988730223No Hit
TTTTTTTTTTTTGTTTTTTTTTTTT132720.17313435405897135No Hit
TTTTTTTTTTTTTTTTTTTTGTTTT132190.1724429646101222No Hit
TTTTTATTTTTTTTTTTTTTTTTTT132030.17223424326707343No Hit
TTTTTTTTGTTTTTTTTTTTTTTTT123530.16114592191760646No Hit
TTTTTTTTTTTTTTTTTTTTTTGGT123120.16061107347604395No Hit
TTTATTTTTTTTTTTTTTTTTTTTT116620.15213176891468683No Hit
TTTTTTATTTTTTTTTTTTTTTTTT115560.15074899001698863No Hit
TTTTTTTTATTTTTTTTTTTTTTTT115540.15072289984910753No Hit
TTTTTTTTTTTTTTGTTTTTTTTTT114050.14877918234196566No Hit
TTTTTTTTTTTTTTTTTTTTTTATA111660.14566140728017435No Hit
TTTTTTTTTTTTTTTTTGTTTTTTT111470.14541355068530393No Hit
TTTTTTTTTTTGTTTTTTTTTTTTT109190.14243927154685865No Hit
TTTTTTTTTTTTTTTTTTGTTTTTT102250.13338598329211737No Hit
TTATTTTTTTTTTTTTTTTTTTTTT100610.1312465895258673No Hit
TTTTTTTTTTGTTTTTTTTTTTTTT100240.13076392142006696No Hit
TTTTTTTTTTTTTTTTTTTTATTTT94450.12321081781848887No Hit
TTTTATTTTTTTTTTTTTTTTTTTT94170.12284555546815348No Hit
TTTTTTTTTTTTTTTTTTTTTCTTT93400.12184108400473119No Hit
TTTTTTTTTTTTTTTTTTTTTTCCC93010.12133232573104977No Hit
TTTTTTTGTTTTTTTTTTTTTTTTT91920.11991041158152989No Hit
TTTTTTTATTTTTTTTTTTTTTTTT91600.1194929688954323No Hit
TTTTTTTTTTTTTTTTTTTGTTTTT90770.1184102269283667No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT88670.11567075930085133No Hit
TTTTTTTTTGTTTTTTTTTTTTTTT88280.11516200102716992No Hit
TTTTTTTTTTTTTATTTTTTTTTTT86790.11321828352002805No Hit
TTTTTTTTTTTTTTTTTTTTTACAA86690.11308783268062256No Hit
TTTTTTTTTTTTTTTTTTTTTTCCA83310.10867859430871687No Hit
TTTTTTTTTTTTTTTTTTTTTAAAT83170.10849596313354919No Hit
TTTTTTTTTTTTTTTTTTTTTTTAG78510.10241695401725316No Hit
TTTTTTTTTTTATTTTTTTTTTTTT77020.10047323651011131No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCCTA502.1534834E-719.018821
TCGCCTA307.6752447E-419.018821
TGGCGTA250.005999789619.018821
TGGCCTA502.1534834E-719.018821
CTCCCGG359.870848E-519.0188181
CGCCTAA307.7093526E-419.0062852
GGCGTAA250.006022680619.0062852
GGCCTAA451.6648737E-619.0062852
GCCCTAA401.2826251E-519.0062832
GCCTAAA759.094947E-1219.0015743
GGCTAAA359.9398145E-519.0015723
GGGTGAA250.006031311619.0015723
TTCCACA451.6711238E-618.99884619
AGTTTAG250.006036313318.99884619
CCCACTC250.00603676818.99859812
ACCCCTC250.00603676818.99859818
TTAAGGT552.8459908E-818.99859816
AAGGTTG307.730354E-418.99859618
AGTGGGG250.006036995418.99847411
TGGTAAG900.018.99822614
Produced by FastQC (version 0.11.5)