FastQCFastQC Report
Wed 28 Aug 2019
H2HY7BGXC_n02_S17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HY7BGXC_n02_S17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7553238
Sequences flagged as poor quality0
Sequence length25
%GC5

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT278236736.8367447179607No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA4302565.6963119658085715No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA1885862.496757019969449No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT1764012.3354354781353375No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG1258091.6656300251627183No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT1076821.4256402353533675No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC989171.3095972879445874No Hit
TTTTTTTTTTTTTTTTTTTTTTGTT846401.1205790152514723No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA812011.0750488730793337No Hit
TTTTTTTTTTTTTTTTTTTTTTATT643880.8524555958649788No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC399660.5291240657318094No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC342240.4531036887755953No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT302660.4007023213090863No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA267220.3537820468519594No Hit
TTTTTTTTTTTTTTTTTTTTTATTT261150.34574575830921783No Hit
TTTTTTTTTTTTTTTTTTTTTGTTT244740.32401997659811593No Hit
TTTTTTTTTTTTTTTTTTTTTTTCT242820.32147802041985174No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA229630.3040153110493804No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA229590.30396235362899987No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC209200.2769673085900378No Hit
TGTTTTTTTTTTTTTTTTTTTTTTT192360.25467223460984545No Hit
TTTTTTTTTTTTTTTTTTTTTTCTT177560.23507798906905886No Hit
TTTTTTTTTTTTTTTTTTTTTAATT172220.22800817344826152No Hit
TTTTTTTTTTTTTTTTTTTTTTACA165680.21934963521604905No Hit
TTGTTTTTTTTTTTTTTTTTTTTTT160960.21310065961114957No Hit
TTTTTTTTTTTTTTTGTTTTTTTTT160620.21265052153791528No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC158630.21001588987398517No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG148610.1967500560686688No Hit
TTTTTGTTTTTTTTTTTTTTTTTTT145900.19316219083788966No Hit
TTTTTTTTTTTTTTTTTTTTTTACC145290.19235459017708695No Hit
TTTGTTTTTTTTTTTTTTTTTTTTT144280.19101741531247923No Hit
TTTTTTTTTTTTTTTTGTTTTTTTT136630.1808893086647078No Hit
TTTTGTTTTTTTTTTTTTTTTTTTT136100.18018762284466608No Hit
TTTTTTTTTTTTGTTTTTTTTTTTT135530.1794329796042439No Hit
TTTTTTTTTTTTTTTTTTTTTTCAA135010.17874453313929736No Hit
TTTTTTGTTTTTTTTTTTTTTTTTT134670.17829439506606307No Hit
TTTTTTTTTTTTTGTTTTTTTTTTT133870.17723524665845297No Hit
TTTTTATTTTTTTTTTTTTTTTTTT132310.1751699072636133No Hit
TTTTTTTTTTTTTTTTTTTTGTTTT132080.1748654020964254No Hit
TTTTTTTTGTTTTTTTTTTTTTTTT121880.1613612598993968No Hit
TTTTTTTTTTTTTTTTTTTTTTGGT120340.15932239921474736No Hit
TTTATTTTTTTTTTTTTTTTTTTTT119140.15773367660333223No Hit
TTTTTTATTTTTTTTTTTTTTTTTT117630.1557345339839682No Hit
TTTTTTTTATTTTTTTTTTTTTTTT116450.15417229008274333No Hit
TTTTTTTTTTTTTTGTTTTTTTTTT112830.14937964353830768No Hit
TTTTTTTTTTTTTTTTTGTTTTTTT108690.14389855052892547No Hit
TTTTTTTTTTTGTTTTTTTTTTTTT108640.14383235375344985No Hit
TTTTTTTTTTTTTTTTTTTTTTATA107630.1424951788888421No Hit
TTATTTTTTTTTTTTTTTTTTTTTT105840.14012533432681454No Hit
TTTTTTTTTTGTTTTTTTTTTTTTT102560.1357828258556132No Hit
TTTTTTTTTTTTTTTTTTGTTTTTT98840.13085778576022628No Hit
TTTTTTTATTTTTTTTTTTTTTTTT95040.12582683082407836No Hit
TTTTATTTTTTTTTTTTTTTTTTTT93490.12377473078433382No Hit
TTTTTTTTTTTTTTTTTTTTTCTTT93020.12315248109486289No Hit
TTTTTTTTTTTTTTTTTTTTATTTT92850.12292741205824574No Hit
TTTTTTTGTTTTTTTTTTTTTTTTT90780.12018686555355465No Hit
TTTTTTTTTTTTTTTTTTTTTTCCC90010.11916743521122994No Hit
TTTTTTTTTGTTTTTTTTTTTTTTT89580.11859814294213952No Hit
TTTTTTTTTTTTTATTTTTTTTTTT89230.11813476551381011No Hit
TTTTTTTTTTTTTTTTTTTGTTTTT87510.11585759643744842No Hit
TTTTTTTTTTTTTTTTTTTTTACAA86570.11461309705850656No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT86160.1140702834996064No Hit
TTTTTTTTTTTTTTTTTTTTTTCCA80340.106364978834243No Hit
TTTTTTTTTTTTTTTTTTTTTAAAT80040.10596779818138923No Hit
TTTTTTTTTTTATTTTTTTTTTTTT79600.10538526655720368No Hit
TTCTTTTTTTTTTTTTTTTTTTTTT76420.10117515163695359No Hit
TTTTTTTTTTTTTTTTATTTTTTTT75950.10055290194748265No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGCTA552.8003342E-819.0254121
TCACCTA307.657362E-419.0254121
TCCCCTA1200.019.0254121
GCCCTAA250.00601419919.010922
CGCCTAA250.00601419919.010922
GGGCTAA502.1629239E-719.010922
GGCCTAA451.6609883E-619.0109182
CCCTCAA250.006029402419.002613
CCCTAAA1450.019.002613
GCCTAAA801.8189894E-1219.0026073
GGCTAAA603.7198333E-919.0026073
CCTAAAC1700.018.9985834
GCTAAAA451.6713384E-618.9985834
TAAGGTT2300.018.9978317
TGGTAAG1800.018.99782814
CACCTCC451.6719732E-618.99782813
ACCTCCC552.8472641E-818.99782814
GGTGAGG552.847446E-818.99770215
GTAAGTT603.7307473E-918.99770216
GTTAAGT307.7327894E-418.99770215
Produced by FastQC (version 0.11.5)