FastQCFastQC Report
Wed 28 Aug 2019
H2HY7BGXC_n02_S16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HY7BGXC_n02_S16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8459794
Sequences flagged as poor quality0
Sequence length25
%GC5

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT303013035.818011644255165No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA4784915.6560597102009815No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT2091852.472696143664964No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA2009532.375388809703877No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG1523121.8004220906561081No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT1186991.4030956309338027No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC1170081.3831069645431082No Hit
TTTTTTTTTTTTTTTTTTTTTTGTT974601.1520375082419265No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA838490.9911470657559747No Hit
TTTTTTTTTTTTTTTTTTTTTTATT678330.8018280350561728No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC453980.5366324522795709No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC405490.479314271718673No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT321250.379737378947998No Hit
TTTTTTTTTTTTTTTTTTTTTTTCT281920.3332468852078431No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA276170.326450029397879No Hit
TTTTTTTTTTTTTTTTTTTTTGTTT275120.3252088644238855No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA274600.3245941922462887No Hit
TTTTTTTTTTTTTTTTTTTTTATTT268650.3175609240603258No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA259030.3061894887747858No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC244690.28923872141567514No Hit
TGTTTTTTTTTTTTTTTTTTTTTTT203670.24075054309833074No Hit
TTTTTTTTTTTTTTTTTTTTTTCTT193440.22865805006599452No Hit
TTTTTTTTTTTTTTTGTTTTTTTTT185070.21876419213044668No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG179860.21260564973567914No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC179740.21246380231007989No Hit
TTTTTTTTTTTTTTTTTTTTTTACA175380.20731001251330705No Hit
TTTTTTTTTTTTTTTTTTTTTAATT174520.20629343929651242No Hit
TTGTTTTTTTTTTTTTTTTTTTTTT169380.20021764123334443No Hit
TTTTTTTTTTTTTTTTTTTTTTACC165520.19565488237656853No Hit
TTTTTGTTTTTTTTTTTTTTTTTTT163810.19363355656177916No Hit
TTTTTTTTTTTTTTTTGTTTTTTTT157390.1860447192922192No Hit
TTTTTTTTTTTTGTTTTTTTTTTTT156070.1844843976106274No Hit
TTTGTTTTTTTTTTTTTTTTTTTTT156010.18441347389782778No Hit
TTTTGTTTTTTTTTTTTTTTTTTTT154500.1826285604590372No Hit
TTTTTTTTTTTTTGTTTTTTTTTTT152980.18083182640144665No Hit
TTTTTTTTTTTTTTTTTTTTGTTTT152820.18064269650064765No Hit
TTTTTTGTTTTTTTTTTTTTTTTTT150390.17777028613226278No Hit
TTTTTTTTTTTTTTTTTTTTTTCAA147170.17396404687868286No Hit
TTTTTATTTTTTTTTTTTTTTTTTT146180.17279380561748903No Hit
TTTTTTTTTTTTTTTTTTTTTTGGT140860.1665052364159222No Hit
TTTTTTTTGTTTTTTTTTTTTTTTT138530.1637510322355367No Hit
TTTTTTTTATTTTTTTTTTTTTTTT130240.15395173925038835No Hit
TTTTTTATTTTTTTTTTTTTTTTTT129680.15328978459759185No Hit
TTTATTTTTTTTTTTTTTTTTTTTT129000.1524859825191961No Hit
TTTTTTTTTTTTTTTTTGTTTTTTT128950.1524268794251964No Hit
TTTTTTTTTTTTTTGTTTTTTTTTT127450.15065378660520576No Hit
TTTTTTTTTTTGTTTTTTTTTTTTT124570.1472494483908237No Hit
TTTTTTTTTTTTTTTTTTTTTTATA119960.14180014312405242No Hit
TTTTTTTTTTTTTTTTTTGTTTTTT116070.13720192241087667No Hit
TTTTTTTTTTGTTTTTTTTTTTTTT114260.13506239040808796No Hit
TTATTTTTTTTTTTTTTTTTTTTTT112810.13334840068209697No Hit
TTTTTTTTTTTTTTTTTTTTTTCCC106080.12539312422973892No Hit
TTTTTTTTTGTTTTTTTTTTTTTTT103270.12207153034695645No Hit
TTTTTTTTTTTTTTTTTTTGTTTTT103020.121776014876958No Hit
TTTTTTTGTTTTTTTTTTTTTTTTT101800.1203338993833656No Hit
TTTTTTTATTTTTTTTTTTTTTTTT101660.12016841072016649No Hit
TTTTATTTTTTTTTTTTTTTTTTTT100230.11847806223177539No Hit
TTTTTTTTTTTTTTTTTTTTTCTTT95190.1125204703566068No Hit
TTTTTTTTTTTTTTTTTTTTATTTT94310.11148025590221226No Hit
TTTTTTTTTTTTTATTTTTTTTTTT93340.11033365587861832No Hit
TTTTTTTTTTTTTTTTTTTTTTTAG91150.10774494036143198No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT91080.1076621960298324No Hit
TTTTTTTTTTTTTTTTTTTTTTCCA89170.1054044578390443No Hit
TTTTTTTTTTTTTTTTTTTTTACAA87700.10366682687545346No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCCTA502.1518463E-719.0202561
TGGGCTC250.005997224719.0202561
TGGGCTA451.6532467E-619.0202561
TCACCTA250.005997224719.0202561
TGGCCTA1000.019.0202561
GGGCTAA359.917818E-519.0070972
CACCTAA250.00602124719.0070952
GCCTAAA1050.019.0023783
CCCGAAA359.936737E-519.0023773
GGCTAAA359.936737E-519.0023773
GCGTAAA250.006029889519.0023753
CCCTCAA250.006029889519.0023753
CCTCAAC250.006035862518.9991194
CGTAAAA250.006035862518.9991194
CCGAAAC307.729016E-418.9991194
CTAAACA451.6714075E-618.9985585
CGAAACC250.00603689318.9985585
CTCAACC250.00603689318.9985585
GTAAAAC451.6714075E-618.9985585
CCCACCT850.018.99833311
Produced by FastQC (version 0.11.5)