FastQCFastQC Report
Wed 28 Aug 2019
H2HY7BGXC_n02_S15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HY7BGXC_n02_S15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8979579
Sequences flagged as poor quality0
Sequence length25
%GC5

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT335179937.3269058605086No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA4945715.50773037355092No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT2176242.4235434645655434No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA2143942.3875729585986156No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG1510991.6826958145810624No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT1291731.4385195564290931No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC1118441.2455372350975473No Hit
TTTTTTTTTTTTTTTTTTTTTTGTT1058731.1790419127667344No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA918091.022419870686588No Hit
TTTTTTTTTTTTTTTTTTTTTTATT767700.854939858539025No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC449200.5002461696700926No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC387650.4317017535009158No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT356010.39646624858470536No Hit
TTTTTTTTTTTTTTTTTTTTTATTT312210.34768890612800446No Hit
TTTTTTTTTTTTTTTTTTTTTGTTT305360.34006048613192225No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA303050.3374879824544113No Hit
TTTTTTTTTTTTTTTTTTTTTTTCT280070.3118965822339778No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA258460.28783086601276076No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA248360.2765831226608731No Hit
TGTTTTTTTTTTTTTTTTTTTTTTT240550.2678856102273837No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC223760.24918762895231503No Hit
TTTTTTTTTTTTTTTTTTTTTTCTT207250.2308014663048234No Hit
TTTTTTTTTTTTTTTGTTTTTTTTT206340.2297880557651979No Hit
TTGTTTTTTTTTTTTTTTTTTTTTT204790.22806191693396763No Hit
TTTTTTTTTTTTTTTTTTTTTAATT201040.22388577460034595No Hit
TTTGTTTTTTTTTTTTTTTTTTTTT187550.20886279857886433No Hit
TTTTTTTTTTTTTTTTTTTTTTACA187110.20837279787838606No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC180040.2004993775320647No Hit
TTTTTGTTTTTTTTTTTTTTTTTTT179670.20008733148848068No Hit
TTTTTTGTTTTTTTTTTTTTTTTTT174980.19486436947656455No Hit
TTTTGTTTTTTTTTTTTTTTTTTTT173370.19307141236799633No Hit
TTTTTTTTTTTTGTTTTTTTTTTTT172550.1921582292443777No Hit
TTTTTTTTTTTTTTTTGTTTTTTTT170170.1895077709099725No Hit
TTTTTTTTTTTTTGTTTTTTTTTTT168500.18764799552406633No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG165660.18448526373007018No Hit
TTTTTTTTTTTTTTTTTTTTTTACC160660.178917073951908No Hit
TTTTTTTTTTTTTTTTTTTTGTTTT160020.17820434566030324No Hit
TTTTTATTTTTTTTTTTTTTTTTTT154640.1722129734590007No Hit
TTTTTTTTGTTTTTTTTTTTTTTTT153800.17127751757626944No Hit
TTTTTTTTTTTTTTTTTTTTTTCAA149770.1667895566150707No Hit
TTTTTTTTTTTTTTTTTTTTTTGGT142980.15922795489632643No Hit
TTTTTTTTTTTTTTGTTTTTTTTTT141610.15770227089711No Hit
TTTTTTTTTTTTTTTTTGTTTTTTT141200.15724567933530068No Hit
TTTTTTTTTTTGTTTTTTTTTTTTT140930.15694499708727994No Hit
TTTTTTTTATTTTTTTTTTTTTTTT136370.15186680800959598No Hit
TTTATTTTTTTTTTTTTTTTTTTTT133870.14908271312051488No Hit
TTTTTTATTTTTTTTTTTTTTTTTT132690.1477686203328686No Hit
TTTTTTTTTTGTTTTTTTTTTTTTT130560.1453965714873715No Hit
TTTTTTTTTTTTTTTTTTGTTTTTT124960.13916019893582984No Hit
TTATTTTTTTTTTTTTTTTTTTTTT118620.13209973429712019No Hit
TTTTTTTTTTTTTTTTTTTTTTATA117080.13038473184544622No Hit
TTTTTTTGTTTTTTTTTTTTTTTTT115030.12810177403639972No Hit
TTTTTTTTTGTTTTTTTTTTTTTTT113370.12625313503004987No Hit
TTTTTTTTTTTTTTTTTTTGTTTTT112740.1255515431180014No Hit
TTTTTTTATTTTTTTTTTTTTTTTT110760.12334653996584918No Hit
TTTTTTTTTTTTTTTTTTTTATTTT108740.12109699129547165No Hit
TTTTTTTTTTTTTTTTTTTTTCTTT107830.12008358075584612No Hit
TTTTATTTTTTTTTTTTTTTTTTTT106270.11834630554505951No Hit
TTTTTTTTTTTTTTTTTTTTTTCCC101170.11266675197133408No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT99690.11101856779699806No Hit
TTTTTTTTTTTTTATTTTTTTTTTT98500.10969333862979545No Hit
TTTTTTTTTTTTTTTTTTTTTACAA95080.1058846968215325No Hit
TTTTTTTTTTTTTTTTTTTTTAAAT94670.1054281052597232No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACCTA250.00598915819.0247041
TGCCCTA1050.019.0247041
TCGCCTA654.802132E-1019.0247041
TCGCCAA250.00598915819.0247041
TGGGCTC451.6495742E-619.0247041
TCACCTA950.019.0247041
TGGCGTA502.1465894E-719.0247041
TGGGATA250.00598915819.0247041
TGGCCTC552.8016075E-819.0247041
TGGCCTA1750.019.0247041
TGCGCTA359.8476856E-519.0247021
TCCGCTA401.271996E-519.0247021
TCACATA307.6594355E-419.0247021
TGCGGTA359.8476856E-519.0247021
GCCCTAA950.019.0101812
CCGCTAA359.905569E-519.010182
GCGGTAA307.6988747E-419.010182
CCCGTAA401.2804001E-519.010182
CCACTAA250.006015633719.010182
GGCCTCA401.2804001E-519.010182
Produced by FastQC (version 0.11.5)