FastQCFastQC Report
Wed 28 Aug 2019
H2HY7BGXC_n02_S14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HY7BGXC_n02_S14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7775055
Sequences flagged as poor quality0
Sequence length25
%GC6

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT278105835.76898169852175No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA3564884.5850222281385795No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT2026262.6061037510345586No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA1520871.9560890566047442No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG1327521.70740914372953No Hit
TTTTTTTTTTTTTTTTTTTTTTGTT1044461.343347410404171No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT1021131.3133411917986433No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC845741.0877607939750908No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA650550.8367143383551628No Hit
TTTTTTTTTTTTTTTTTTTTTTATT649300.8351066326861997No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC324760.4176947944419686No Hit
TTTTTTTTTTTTTTTTTTTTTGTTT308770.3971290235245924No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC292810.37660183754327137No Hit
TTTTTTTTTTTTTTTTTTTTTATTT271260.3488849918103473No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT267160.3436117172161483No Hit
TGTTTTTTTTTTTTTTTTTTTTTTT226690.29156063847779856No Hit
TTTTTTTTTTTTTTTTTTTTTTTCT223990.2880879942328382No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA214450.2758179845673117No Hit
TTTTTTTTTTTTTTTGTTTTTTTTT213040.27400449257272136No Hit
TTGTTTTTTTTTTTTTTTTTTTTTT192520.2476123963110229No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA190900.24552880976404667No Hit
TTTTTGTTTTTTTTTTTTTTTTTTT188010.24181179425740396No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA185290.23831342672174022No Hit
TTTGTTTTTTTTTTTTTTTTTTTTT182190.2343263166627117No Hit
TTTTTTTTTTTTGTTTTTTTTTTTT180480.23212697530757015No Hit
TTTTTTTTTTTTTTTTGTTTTTTTT178930.23013342027805592No Hit
TTTTTTTTTTTTTTTTTTTTTTCTT175020.2251045169455393No Hit
TTTTTTTTTTTTTGTTTTTTTTTTT174880.22492445391061544No Hit
TTTTTTGTTTTTTTTTTTTTTTTTT174670.22465435935822964No Hit
TTTTTTTTTTTTTTTTTTTTGTTTT174070.22388266063712733No Hit
TTTTGTTTTTTTTTTTTTTTTTTTT173270.22285372900899092No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC171940.22114313017721418No Hit
TTTTTTTTGTTTTTTTTTTTTTTTT163080.20974771239560364No Hit
TTTTTTTTTTTTTTTTTTTTTAATT154990.19934264130607435No Hit
TTTTTTTTTTTTTTTTTGTTTTTTT149110.19177999383927188No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG148170.19057099917621162No Hit
TTTTTTTTTTTTTTGTTTTTTTTTT145130.18666105898929333No Hit
TTTTTTTTTTTGTTTTTTTTTTTTT141010.1813620611043909No Hit
TTTTTTTTTTTTTTTTTTTTTTGGT135010.17364507389336795No Hit
TTTTTTTTTTGTTTTTTTTTTTTTT132760.17075120368923435No Hit
TTTTTTTTTTTTTTTTTTTTTTACA132590.17053255571825537No Hit
TTTTTTTTTTTTTTTTTTGTTTTTT131920.16967082547969115No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC131690.16937500763660193No Hit
TTTTTATTTTTTTTTTTTTTTTTTT128750.16559368390320067No Hit
TTTTTTTGTTTTTTTTTTTTTTTTT120610.1551243045869129No Hit
TTTTTTTTTTTTTTTTTTTTTTACC119520.15372238524357706No Hit
TTTTTTTTTTTTTTTTTTTGTTTTT116790.15021115606256163No Hit
TTTTTTTTTGTTTTTTTTTTTTTTT116660.15004395467298945No Hit
TTTTTTTTATTTTTTTTTTTTTTTT112260.1443848307182393No Hit
TTTTTTATTTTTTTTTTTTTTTTTT111040.1428157099853313No Hit
TTTATTTTTTTTTTTTTTTTTTTTT110830.1425456154329455No Hit
TTTTTTTTTTTTTTTTTTTTTTCAA109460.1407835700197619No Hit
TTATTTTTTTTTTTTTTTTTTTTTT97070.12484799142899954No Hit
TTTTTTTTTTTTTTTTTTTTATTTT94140.12107952934095No Hit
TTTTTTTTTTTTTTTTTTTTTCTTT92620.11912455924749087No Hit
TTTTTTTTTTTTTTTTTTTTTTATA89020.1144943669208771No Hit
TTTTTTTATTTTTTTTTTTTTTTTT88820.11423713401384299No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT87480.11251367353671453No Hit
TTTTATTTTTTTTTTTTTTTTTTTT86100.11073876647817926No Hit
TTTTTTTTTTTTTATTTTTTTTTTT82160.10567127820960752No Hit
TTTTTTTTTTTTTTTTTTTTTTAGT80850.10398640266853418No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCTA401.2745077E-519.020291
TCGCCTA654.820322E-1019.0202881
TGGGCTA900.019.0202881
TGGCGTA706.366463E-1119.0202881
TGCGGTA250.005997120419.0202881
TGGCCTA1250.019.0202881
GCCCTAA759.094947E-1219.009642
CGCCTAA603.7016434E-919.009642
GGCGTAA552.8270733E-819.009642
CCGCTAA401.2806773E-519.009642
GGCCTAA1200.019.009642
GGGCTAA706.366463E-1119.0096382
GCCTAAA1750.019.003283
CGCTAAA502.1720916E-719.003283
CCGTAAA250.00602818819.003283
GGCTAAA654.8748916E-1019.003283
GCGTAAA552.8379873E-819.003283
CGGTAAA250.00602818819.003283
CCTAACC451.6714093E-618.9985144
GCTAAAC603.7289283E-918.9985144
Produced by FastQC (version 0.11.5)