FastQCFastQC Report
Wed 28 Aug 2019
H2HY7BGXC_n02_S13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HY7BGXC_n02_S13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9068995
Sequences flagged as poor quality0
Sequence length25
%GC6

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT323240635.64238374814409No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA4833205.329366704910522No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT2301392.5376461228614637No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA2024722.232573730606313No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG1630421.7977956763676681No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT1270621.4010593235523892No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC1205031.3287359845275026No Hit
TTTTTTTTTTTTTTTTTTTTTTGTT1115931.2304891556341138No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA841970.9284049665922188No Hit
TTTTTTTTTTTTTTTTTTTTTTATT739720.8156581848374599No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC463990.5116222911138445No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC422890.4663030468094866No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT345780.3812770874832327No Hit
TTTTTTTTTTTTTTTTTTTTTGTTT313860.346080243731527No Hit
TTTTTTTTTTTTTTTTTTTTTTTCT307740.33933197669642556No Hit
TTTTTTTTTTTTTTTTTTTTTATTT299690.3304555796976401No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA272830.30083818548802815No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA264360.29149867212408875No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA255400.28161885633413625No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC242480.2673725148155887No Hit
TGTTTTTTTTTTTTTTTTTTTTTTT226710.24998359796206746No Hit
TTTTTTTTTTTTTTTTTTTTTTCTT216380.23859314069530307No Hit
TTTTTTTTTTTTTTTGTTTTTTTTT207130.22839355408179185No Hit
TTTTTTTTTTTTTTTTTTTTTAATT192740.21252630528520527No Hit
TTGTTTTTTTTTTTTTTTTTTTTTT188790.2081708061367329No Hit
TTTTTGTTTTTTTTTTTTTTTTTTT182620.20136740620101787No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC180310.19882026619267076No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG180210.1987100003914436No Hit
TTTTTTTTTTTTTTTTTTTTTTACA178360.19667008306874134No Hit
TTTTTTTTTTTTTTTTTTTTGTTTT172360.19005413499511248No Hit
TTTTTTTTTTTTTTTTGTTTTTTTT170730.18825680243510995No Hit
TTTGTTTTTTTTTTTTTTTTTTTTT169760.18718722416320663No Hit
TTTTTTTTTTTTGTTTTTTTTTTTT167970.18521346632124067No Hit
TTTTTTGTTTTTTTTTTTTTTTTTT167660.1848716423374365No Hit
TTTTTTTTTTTTTGTTTTTTTTTTT166570.1836697451040606No Hit
TTTTTTTTTTTTTTTTTTTTTTACC166470.18355947930283345No Hit
TTTTGTTTTTTTTTTTTTTTTTTTT159770.1761716706206145No Hit
TTTTTTTTGTTTTTTTTTTTTTTTT158210.17445152412147102No Hit
TTTTTTTTTTTTTTTTTTTTTTGGT150310.1657405258245263No Hit
TTTTTATTTTTTTTTTTTTTTTTTT150310.1657405258245263No Hit
TTTTTTTTTTTTTTTTTTTTTTCAA148840.16411961854648724No Hit
TTTTTTTTTTTTTTTTTGTTTTTTT148530.16377779456268307No Hit
TTTTTTTTTTTTTTGTTTTTTTTTT143590.15833066398206197No Hit
TTTTTTTTTTTGTTTTTTTTTTTTT135150.14902423035849066No Hit
TTTATTTTTTTTTTTTTTTTTTTTT130290.14366531241885125No Hit
TTTTTTATTTTTTTTTTTTTTTTTT130260.14363223267848313No Hit
TTTTTTTTATTTTTTTTTTTTTTTT130250.1436212060983604No Hit
TTTTTTTTTTTTTTTTTTGTTTTTT130090.14344478081639697No Hit
TTTTTTTTTTGTTTTTTTTTTTTTT129150.14240828228486177No Hit
TTTTTTTTTTTTTTTTTTTGTTTTT117660.12973874172386246No Hit
TTTTTTTTTTTTTTTTTTTTTTATA115630.12750034595895135No Hit
TTATTTTTTTTTTTTTTTTTTTTTT114950.12675053851060675No Hit
TTTTTTTGTTTTTTTTTTTTTTTTT111860.12334332525268787No Hit
TTTTTTTTTGTTTTTTTTTTTTTTT109910.12119314212875848No Hit
TTTTTTTTTTTTTTTTTTTTTTCCC106660.11760950358887616No Hit
TTTTTTTATTTTTTTTTTTTTTTTT105990.11687072272065428No Hit
TTTTTTTTTTTTTTTTTTTTTCTTT104740.11549240020531493No Hit
TTTTATTTTTTTTTTTTTTTTTTTT103310.1139155992477667No Hit
TTTTTTTTTTTTTTTTTTTTATTTT103110.11369506764531241No Hit
TTTTTTTTTTTTTTTTTTTTTTTAG97830.107873033340519No Hit
TTTTTTTTTTTTTTTTTTTTTTCCT96710.10663805636677492No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT96710.10663805636677492No Hit
TTTTTTTTTTTTTATTTTTTTTTTT95660.10548026545388986No Hit
TTTTTTTTTTTTTTTTTTTTTAAAT92150.10160993583081697No Hit
TTTTTTTTTTTTTTTTTTTTTTAGT92110.10156582951032611No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCTA250.005995046319.021471
TGGGCTA850.019.021471
TCACCTA250.005995046319.021471
TGGCGTA307.6682057E-419.021471
GGCGTAA250.006016870519.0095062
GGCCTAA1000.019.0095062
CACCTAA250.006016870519.0095062
GGGCTAA654.8567017E-1019.0095042
GGCTAAA850.019.0036353
GCCTCAA250.00602761519.0036333
CCCTAAC401.2842102E-519.0036333
GCAAAAC250.00603510718.9995484
GCTAAAC401.2865939E-518.9995484
CCTCAAC502.1766937E-718.9995484
GGTAAAC359.948197E-518.9995484
GTAAACA250.00603683718.9986065
CTCAACC603.7289283E-918.9986045
GTAAAAC603.7289283E-918.9986045
TGAAACC250.00603818318.9978716
TGAAAAC250.00603818318.9978716
Produced by FastQC (version 0.11.5)