FastQCFastQC Report
Wed 28 Aug 2019
H2HY7BGXC_n02_S12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HY7BGXC_n02_S12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8730021
Sequences flagged as poor quality0
Sequence length25
%GC6

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT301324934.515942172418605No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA5180495.934109436850152No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA2164482.479352569713177No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT2064282.3645762135050994No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG1534151.7573268151359547No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT1346641.5425392447509576No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC1289721.4773389434000215No Hit
TTTTTTTTTTTTTTTTTTTTTTGTT971601.1129411945286272No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA903971.0354728814512588No Hit
TTTTTTTTTTTTTTTTTTTTTTATT754350.864087268518598No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC530560.607741951594389No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC460510.5275015947842507No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT361240.41379052810984074No Hit
TTTTTTTTTTTTTTTTTTTTTTTCT319990.3665397826648985No Hit
TTTTTTTTTTTTTTTTTTTTTATTT293230.3358869354380705No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA293050.33568075036703804No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA283320.3245353018051159No Hit
TTTTTTTTTTTTTTTTTTTTTGTTT273920.3137678592067534No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA273120.31285148111327565No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC270460.30980452395246244No Hit
TTTTTTTTTTTTTTTTTTTTTTCTT215910.24731899270345395No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC209950.24049197590704532No Hit
TTTTTTTTTTTTTTTTTTTTTAATT195950.22445535927118615No Hit
TTTTTTTTTTTTTTTTTTTTTTACA193650.22182077225243788No Hit
TTTTTTTTTTTTTTTTTTTTTTACC190540.21825835241404343No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG182210.20871656551570725No Hit
TGTTTTTTTTTTTTTTTTTTTTTTT180640.2069181735072573No Hit
TTTTTTTTTTTTTTTGTTTTTTTTT177540.20336720839503136No Hit
TTTTTTTTTTTTTTTTTTTTTTCAA158660.1817406853889584No Hit
TTGTTTTTTTTTTTTTTTTTTTTTT154120.17654023970847263No Hit
TTTTTGTTTTTTTTTTTTTTTTTTT149560.17131688457564992No Hit
TTTGTTTTTTTTTTTTTTTTTTTTT148660.17028595922048756No Hit
TTTTTTTTTTTTTGTTTTTTTTTTT145600.16678081301293549No Hit
TTTTTTTTTTTTTTTTGTTTTTTTT145430.16658608266807146No Hit
TTTTTATTTTTTTTTTTTTTTTTTT140180.16057235142962428No Hit
TTTTTTTTTTTTTTTTTTTTGTTTT139680.15999961512120073No Hit
TTTTTTTTTTTTTTTTTTTTTTGGT139000.15922069374174472No Hit
TTTTTTTTTTTTGTTTTTTTTTTTT138440.15857922907631036No Hit
TTTTTTGTTTTTTTTTTTTTTTTTT136630.15650592363981713No Hit
TTTTGTTTTTTTTTTTTTTTTTTTT135250.15492517142856815No Hit
TTTTTTTTTTTTTTTTTTTTTTATA134290.15382551771639497No Hit
TTTATTTTTTTTTTTTTTTTTTTTT128660.14737650688354587No Hit
TTTTTTTTTTTTTTTTTTTTTTCCC123250.14117950002640314No Hit
TTTTTTTTGTTTTTTTTTTTTTTTT122980.14087022241985442No Hit
TTTTTTATTTTTTTTTTTTTTTTTT122540.14036621446844172No Hit
TTTTTTTTATTTTTTTTTTTTTTTT122240.14002257268338758No Hit
TTTTTTTTTTTTTTTTTGTTTTTTT120810.13838454684129625No Hit
TTTTTTTTTTTTTTGTTTTTTTTTT119830.13726198367678613No Hit
TTTTTTTTTTTGTTTTTTTTTTTTT114300.13092752010562175No Hit
TTATTTTTTTTTTTTTTTTTTTTTT114110.1307098803084208No Hit
TTTTTTTTTTTTTTTTTTGTTTTTT111070.12722764355320565No Hit
TTTTTTTTTTTTTTTTTTTTTCTTT105180.12048080983997633No Hit
TTTTATTTTTTTTTTTTTTTTTTTT103510.1185678705698417No Hit
TTTTTTTATTTTTTTTTTTTTTTTT103100.1180982267969344No Hit
TTTTTTTTTTGTTTTTTTTTTTTTT103040.11802949843992357No Hit
TTTTTTTTTTTTTTTTTTTGTTTTT101700.11649456513334847No Hit
TTTTTTTTTTTTTTTTTTTTTTCCA101180.115898919372588No Hit
TTTTTTTTTTTTTTTTTTTTATTTT100260.11484508456508868No Hit
TTTTTTTTTTTTTTTTTTTTTTTAG96020.10998828066965703No Hit
TTTTTTTTTTTTTATTTTTTTTTTT95860.10980500505096151No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT95690.1096102747060975No Hit
TTTTTTTGTTTTTTTTTTTTTTTTT94490.108235707565881No Hit
TTTTTTTTTTTTTTTTTTTTTACAA91650.10498256533403529No Hit
TTTTTTTTTGTTTTTTTTTTTTTTT91280.10455874046580187No Hit
TTTTTTTTTTTTTTTTTTTTTTCCT89930.10301235243305829No Hit
TTTTTTTTTTTTTTTTTTTTTAAAT89790.1028519862666997No Hit
TTTTTTTTTTTTTTTTTTTTTTACT88140.10096195644890202No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCTC250.00599715619.0203021
TGCCCTC250.00599715619.0203021
TCGCCTA401.27453E-519.0203021
TGGGCTA801.8189894E-1219.0203021
TGGCGTA401.27453E-519.0203021
TGGCCTC401.27453E-519.0203021
TCGGGTA250.00599715619.0203021
TGCCCTA950.019.02031
TGGCCTA1250.019.02031
GCCCTAA759.094947E-1219.0074392
GGCGTAA250.00602063319.0074392
CGGGTAA250.00602063319.0074392
GGCCTCT250.00602063319.0074392
GGCCTAA1300.019.0074372
GGGCTAA654.8748916E-1019.0074372
CGCTAAA502.1741107E-719.0016673
GGCTAAA654.8930815E-1019.0016673
GCGTAAA359.939636E-519.0016673
CCCTATA250.006031202619.0016673
ACCACCG250.006036592618.9987311
Produced by FastQC (version 0.11.5)