FastQCFastQC Report
Wed 28 Aug 2019
H2HY7BGXC_n02_S11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HY7BGXC_n02_S11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7408825
Sequences flagged as poor quality0
Sequence length25
%GC5

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT270166936.465552904812846No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA4701806.346215493010026No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA1991892.6885369812352162No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT1634652.2063552587623545No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG1233931.6654867674698755No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT1183491.5974057964657014No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC1014751.3696503831579232No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA841281.1355106916413873No Hit
TTTTTTTTTTTTTTTTTTTTTTGTT764091.0313241303445553No Hit
TTTTTTTTTTTTTTTTTTTTTTATT667410.9008311034475778No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC451070.6088279855442665No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC356370.48100744719979216No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT317450.42847550050109157No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA269890.36428178557328594No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA265840.35881533171589286No Hit
TTTTTTTTTTTTTTTTTTTTTATTT253800.3425644417299639No Hit
TTTTTTTTTTTTTTTTTTTTTTTCT242820.32774427793880945No Hit
TTTTTTTTTTTTTTTTTTTTTGTTT220930.29819843227502335No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA218200.2945136374526325No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC217040.29294793708853967No Hit
TGTTTTTTTTTTTTTTTTTTTTTTT185860.2508629910950792No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC180910.24418176971382102No Hit
TTTTTTTTTTTTTTTTTTTTTTCTT177830.24002456529881594No Hit
TTTTTTTTTTTTTTTTTTTTTTACA173900.2347200804446049No Hit
TTTTTTTTTTTTTTTTTTTTTAATT171320.23123774687619156No Hit
TTTTTTTTTTTTTTTTTTTTTTACC156190.21081615505832574No Hit
TTGTTTTTTTTTTTTTTTTTTTTTT154560.2086160760984367No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG149510.20179988054786013No Hit
TTTTTTTTTTTTTTTGTTTTTTTTT143580.1937959123072822No Hit
TTTGTTTTTTTTTTTTTTTTTTTTT139410.18816748944670714No Hit
TTTTTGTTTTTTTTTTTTTTTTTTT134820.18197217507499502No Hit
TTTTTTTTTTTTTTTTTTTTTTCAA134600.18167523190249468No Hit
TTTTTATTTTTTTTTTTTTTTTTTT133410.18006903928760631No Hit
TTTTGTTTTTTTTTTTTTTTTTTTT129670.17502100535510015No Hit
TTTTTTGTTTTTTTTTTTTTTTTTT127750.1724295013041879No Hit
TTTTTTTTTTTTGTTTTTTTTTTTT121850.16446602531440546No Hit
TTTTTTTTTTTTTTTTTTTTTTATA120720.16294081720110815No Hit
TTTTTTTTTTTTTGTTTTTTTTTTT120490.16263037661167595No Hit
TTTTTTTTTTTTTTTTGTTTTTTTT119620.16145610133860633No Hit
TTTATTTTTTTTTTTTTTTTTTTTT117230.15823021869189785No Hit
TTTTTTATTTTTTTTTTTTTTTTTT116180.1568129899140552No Hit
TTTTTTTTATTTTTTTTTTTTTTTT115690.1561516164843953No Hit
TTTTTTTTTTTTTTTTTTTTGTTTT115400.15576019139337208No Hit
TTTTTTTTGTTTTTTTTTTTTTTTT109050.1471893316416571No Hit
TTTTTTTTTTTTTTTTTTTTTTGGT106340.14353153165312987No Hit
TTATTTTTTTTTTTTTTTTTTTTTT102860.1388344305608514No Hit
TTTTTTTTTTTTTTGTTTTTTTTTT100480.13562204533107478No Hit
TTTTTTTTTTTGTTTTTTTTTTTTT99190.1338808785468681No Hit
TTTTTTTTTTTTTTTTTTTTTTCCC98040.1323286755997071No Hit
TTTTTTTTTTTTTTTTTGTTTTTTT97640.1317887789224337No Hit
TTTTTTTATTTTTTTTTTTTTTTTT96360.1300611095551589No Hit
TTTTATTTTTTTTTTTTTTTTTTTT96200.12984515088424953No Hit
TTTTTTTTTTGTTTTTTTTTTTTTT90750.12248905865639964No Hit
TTTTTTTTTTTTTTTTTTGTTTTTT90440.12207063873151276No Hit
TTTTTTTTTTTTTTTTTTTTATTTT89140.12031597453037424No Hit
TTTTTTTTTTTTTATTTTTTTTTTT88160.11899322767105446No Hit
TTTTTTTTTTTTTTTTTTTTTTCCA87940.11869628449855409No Hit
TTTTTTTTTTTTTTTTTTTTTCTTT87810.11852081807844023No Hit
TTTTTTTTTTTTTTTTTTTTTACAA85850.11587532435980064No Hit
TTTTTTTGTTTTTTTTTTTTTTTTT85230.1150384845100269No Hit
TTTTTTTTTGTTTTTTTTTTTTTTT81820.11043586533627126No Hit
TTTTTTTTTTTTTTTTTTTGTTTTT81430.10990946607592972No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT81230.10963951773729301No Hit
TTTTTTTTTTTTTTTTATTTTTTTT78370.10577925649478831No Hit
TTTTTTTTTTATTTTTTTTTTTTTT77120.10409207937830897No Hit
TTTTTTTTTTTTTTTTTTTTTAAAT76930.10383562845660411No Hit
TTTTTTTTTTTATTTTTTTTTTTTT75240.10155456499512405No Hit
TTTTTTTTTTTTATTTTTTTTTTTT74520.10058275097603196No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCCTA359.8528966E-519.0233171
TGGGCTA307.6630176E-419.0233171
TGGCCTA603.6689016E-919.0233171
GGCCTAA552.8279828E-819.0090582
GCCCTAA359.909764E-519.0090562
GGGCTAA250.00601759219.0090562
GCCTAAA552.8396244E-819.0022563
CGCTAAA250.006030042719.0022543
GGCTAAA401.2849625E-519.0022543
GCTAAAC307.730139E-418.9986634
GCTAAAA359.95144E-518.9986634
CTAAACC1800.018.9982785
AGTGGGG250.0060375718.99815211
GTAAGTT401.287508E-518.99789416
GGTAAGG900.018.99789415
CATCCCC359.954533E-518.99789215
TGGTGAG401.2876577E-518.99763714
TTTCCGA359.956596E-518.99738119
AGTTTGC359.956596E-518.99738119
AAGGTTG451.6725571E-618.99712618
Produced by FastQC (version 0.11.5)