FastQCFastQC Report
Wed 28 Aug 2019
H2HY7BGXC_n02_S10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HY7BGXC_n02_S10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4540513
Sequences flagged as poor quality0
Sequence length25
%GC6

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT150831233.21897767939438No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA2441235.376551063723416No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA1154742.5431928066277973No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT845511.8621464138523554No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT673221.4826958980185718No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG605291.3330872524756563No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA558611.230279486040454No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC536341.1812321647355706No Hit
TTTTTTTTTTTTTTTTTTTTTTGTT466601.0276371854898334No Hit
TTTTTTTTTTTTTTTTTTTTTTATT460351.0138722210463884No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC256050.5639230633190567No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC216280.4763338415725272No Hit
TTTTTTTTTTTTTTTTTTTTTATTT214230.47181893323507723No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT209120.4605646983061165No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA208440.4590670701746697No Hit
TTTTTTTTTTTTTTTTTTTTTTTCT143880.31688049345965974No Hit
TTTTTTTTTTTTTTTTTTTTTAATT143640.31635191882503144No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA136170.2999000333222259No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA134060.2952529813261189No Hit
TTTTTTTTTTTTTTTTTTTTTGTTT129060.28424100977136285No Hit
TTTTTTTTTTTTTTTTTTTTTTCTT126270.278096329643809No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC120000.2642873173141449No Hit
TTTTTTTTTTTTTTTTTTTTTTACA114850.25294498661274617No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC114360.25186581340038006No Hit
TTTTTTTTTTTTTTTTTTTTTTACC103660.22830019427320217No Hit
TTTTTTTTTTTTTTTTTTTTTTCAA99750.21968883251738294No Hit
TGTTTTTTTTTTTTTTTTTTTTTTT92680.2041179047389579No Hit
TTTTTTTTTTTTTTTTTTTTATTTT81770.18008978280648025No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG80850.17806358004040512No Hit
TTTTTATTTTTTTTTTTTTTTTTTT79130.17427546182556905No Hit
TTTTTTTTTTTTTTTTTTTTTCTTT77400.17046531966762346No Hit
TTTTTTTTTTTTTTTTTTTTTTATA74570.16423254376763152No Hit
TTGTTTTTTTTTTTTTTTTTTTTTT74430.1639242085640984No Hit
TTTTTTTTTTTTTTTTTTTTTTCCC72610.15991585091816718No Hit
TTTTTTTTATTTTTTTTTTTTTTTT67900.14954257371358698No Hit
TTTTTTTTTTTTTTTTTTTTTACAA67590.14885983147719212No Hit
TTTGTTTTTTTTTTTTTTTTTTTTT67270.14815506529768774No Hit
TTTTTTATTTTTTTTTTTTTTTTTT66530.14652529350758384No Hit
TTTATTTTTTTTTTTTTTTTTTTTT64960.14306753443939044No Hit
TTTTTTTTTTTTTTTGTTTTTTTTT64680.1424508640323241No Hit
TTTTTTTTTTTTTTTTTTTTTAAAT64620.14231872037366702No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT64540.14214252882879094No Hit
TTTTGTTTTTTTTTTTTTTTTTTTT63270.13934548805388292No Hit
TTTTTTTTTTTTTTTTTTTTTTCCA62850.1384204824432834No Hit
TTTTTGTTTTTTTTTTTTTTTTTTT61980.13650439939275583No Hit
TTTTTTTTTTTTTTTTTTTTGTTTT60380.1329805684952339No Hit
TTTTTTGTTTTTTTTTTTTTTTTTT59530.1311085333309254No Hit
TTTTTTTTTTTTTTTTTTTTTTGGT57470.1265716010503659No Hit
TTTTTTTTTTTTTTTTTTTTAATTT57090.12573469121220446No Hit
TTATTTTTTTTTTTTTTTTTTTTTT56990.12551445178110932No Hit
TTTTTTTTTTTTTGTTTTTTTTTTT56170.12370848844612933No Hit
TTCTTTTTTTTTTTTTTTTTTTTTT54160.1192816758811174No Hit
TTTTTTTATTTTTTTTTTTTTTTTT53490.11780607169278008No Hit
TTTTATTTTTTTTTTTTTTTTTTTT53180.1171233294563852No Hit
TCTTTTTTTTTTTTTTTTTTTTTTT53130.11701320974083765No Hit
TTTTTTTTTTTTGTTTTTTTTTTTT53050.11683701819596155No Hit
TTTTTTTTTTTTTTTTTTTTAAAAA52470.11555962949560987No Hit
TTTTTTTTTTTTTTTTGTTTTTTTT52110.11476676754366742No Hit
TTTTTTTTGTTTTTTTTTTTTTTTT51580.11359949855886328No Hit
TTTTTTTTTTTTTTTTTTTTTACCA50670.11159531973589769No Hit
TTTTTTTTTTTTTTTTTTTTTTCCT50110.11036197892176501No Hit
TTTTTTTTTTTTTATTTTTTTTTTT47690.10503218468926308No Hit
TTTTTTTTTTTTTTGTTTTTTTTTT47190.10393098753378749No Hit
TTTTTTTTTTTTTTTTTTTTTTACT46950.10340241289915919No Hit
TTTTTTTTTTTTTTTTTTTTTTAGT46840.10316014952495456No Hit
TTTTCTTTTTTTTTTTTTTTTTTTT45430.10005477354651335No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCTA401.2736355E-519.021471
TGCCCAA759.094947E-1219.021471
TGGGCTA1550.019.021471
TCACCTA759.094947E-1219.021471
TGCGGTA401.2736355E-519.021471
TGTCCTA451.6518934E-619.0214671
TGCCGTA250.005994597419.0214671
TCGGCTA359.859173E-519.0214671
TCGCCTA950.019.0214671
TCGCCAA359.859173E-519.0214671
TCCGCTA307.667401E-419.0214671
TGGCGTA603.6725396E-919.0214671
TGGCCTA2900.019.0214671
GTCCTAA359.912693E-519.0080572
CGGCTAA359.912693E-519.0080572
GGGCTCC359.912693E-519.0080572
GCCCTCA250.006019072619.0080552
CGCCTAA1000.019.0080552
GGCGTAA654.8567017E-1019.0080552
GCGGTAA401.2814067E-519.0080552
Produced by FastQC (version 0.11.5)