FastQCFastQC Report
Wed 28 Aug 2019
H2HY7BGXC_n02_Lu_RNAseq_Aug2019_Sample_9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HY7BGXC_n02_Lu_RNAseq_Aug2019_Sample_9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95
Sequences flagged as poor quality0
Sequence length25
%GC36

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTTTTTTGGGGTTTTTGGGGGGG55.263157894736842No Hit
TTTTTTTTTTTTTTTTTTTTTTTTT55.263157894736842No Hit
GGGGGTTTTGGGGTTTTTGGGGGGG44.2105263157894735No Hit
TTTTTGTGTGTTTTTTGTGTTTTTG22.1052631578947367No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT22.1052631578947367No Hit
TTTGTTTTGTTTGGTGTTTTTTGTT22.1052631578947367No Hit
TGCTTGCTTTGTTTGTGGTGTTGGT11.0526315789473684No Hit
CACCCACACCCCCACCCCCCCCTGA11.0526315789473684No Hit
TTTTTTTTTTTTTTTGTTTTTTTGG11.0526315789473684No Hit
TTTTTTTTTTTTTTTTTTTGTTTTG11.0526315789473684No Hit
TGGGTGGGGGTTGTTGGGGTGGGGG11.0526315789473684No Hit
TTTGGTTTGTGTGTGTTGTTTGTTT11.0526315789473684No Hit
TTTTTTTTTTTTGTTTTTGTTTGGG11.0526315789473684No Hit
CGCGTGGCGGTCGGTCTGTGGGGGG11.0526315789473684No Hit
TGTGGTTGGTTTGGTTGTTTTTGTT11.0526315789473684No Hit
TTTTTTGTTTTTGTTTGTTTTTGTG11.0526315789473684No Hit
TTTTGTGTGGTGTGTTGTTTTTTTT11.0526315789473684No Hit
TTGGGGGGGGTTGGGGGGTGGTGGG11.0526315789473684No Hit
TTGTTTTTTTTTTTTGGGTTTTGGG11.0526315789473684No Hit
ATTATTAAAAATAATTACAAAATAA11.0526315789473684No Hit
TTTGTGGGTGTGTTTGTTGGTTTGG11.0526315789473684No Hit
TTGGTGTTTTTTGTGGTTTTTGGGG11.0526315789473684No Hit
TTTGTTTTTTTTTTTTTTTTTTTTG11.0526315789473684No Hit
TTTTTTTTTTTGGTTTTTGTTTTTT11.0526315789473684No Hit
GGGTTTTTTGGGGTTTTTGTGGGGG11.0526315789473684No Hit
TTTTTTGTTGTTTTTGTTGTTTTGT11.0526315789473684No Hit
ATTTTTGGGTGTTTGTTTGTTGTGT11.0526315789473684No Hit
GGGTTTTTTGGGGTTTTTGGGGTTG11.0526315789473684No Hit
TTTTTTTTTTTTAAATTATTTAAAC11.0526315789473684No Hit
TTTTTTTTTTTGTTTTTTTTGGGGG11.0526315789473684No Hit
TGTTTTTTTGTTTTTTTTTTTTGTA11.0526315789473684No Hit
TTGGGGGGGGGGGGGGGGGGGGGGG11.0526315789473684No Hit
TTTTTTTTGTTTTTTGTTTTTTTTT11.0526315789473684No Hit
TTTTTTGTTTTTTTTTTTGTTTTTT11.0526315789473684No Hit
TTGGGGGGGGGTTGGGGGGGGGGGG11.0526315789473684No Hit
TTTTTTTTTTGGTTTGTGTTTTTGT11.0526315789473684No Hit
CCGCGCCCGGGGGGGGGGGGGGTGG11.0526315789473684No Hit
TTTGGTGGGGGGGGGGGGGGGTTTG11.0526315789473684No Hit
CGGGGGGGGGGGTGGGGGCGGTTGG11.0526315789473684No Hit
TTTTGTTTTTGGTGTTTTGTGGGGG11.0526315789473684No Hit
TTTTTTTTTTTTTTTTTTTTGTAAA11.0526315789473684No Hit
CCGGCCCCGTCTTTTGCCCGCTATT11.0526315789473684No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA11.0526315789473684No Hit
GCCGGGTTGGGGGGGTTGTTTTTGG11.0526315789473684No Hit
GCCGGGTTGGGGGGGGTGTTGTGGG11.0526315789473684No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG11.0526315789473684No Hit
TTTTTGTTTTTTTTTTTTTTTTTGT11.0526315789473684No Hit
GGGTTTTGGGGGGGGGGGGGGTTTG11.0526315789473684No Hit
GTGTTTTGTTGTTTTTTGTTTTTTG11.0526315789473684No Hit
TTTTTTTTTTTTTTTGTTTTTTTAT11.0526315789473684No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA11.0526315789473684No Hit
TGGTTTGGTTTTTTTGTTTTGTTGG11.0526315789473684No Hit
TTGTGTTTTTTGTGGTTGTGTTTGG11.0526315789473684No Hit
ACAGGGTTACAACTATTTCTTTTTG11.0526315789473684No Hit
TGAATGATTATCCTTTATCCCCTAT11.0526315789473684No Hit
TTTTTTATTTTTTTTTTTTTTTTTA11.0526315789473684No Hit
TTTTGTGTTTTTTTGGTTGTTTTGT11.0526315789473684No Hit
TGTGGGGGGGGGGGGGTGTGTTTGT11.0526315789473684No Hit
AGGGTTTGGGCGGGCTTGGCGTTTG11.0526315789473684No Hit
ACTCTTTTTCTGCGGGGCGGTTGCT11.0526315789473684No Hit
TTTTGTGTGTTTTTGGTGGTTTGGG11.0526315789473684No Hit
GGGTTTAAAAAGGGGGGGGGGTGGG11.0526315789473684No Hit
TTTGTTTTTTTGTGTGTTTTGTTTT11.0526315789473684No Hit
TCCCCCCACCCCCCCCCCCCCCACC11.0526315789473684No Hit
TTTTTTTTTTTGGGTTTTTTTTGTT11.0526315789473684No Hit
TTCATTTCATCTATCAAACTAATAA11.0526315789473684No Hit
TTTGTGTTTGGGGGGTTTTTTTGGG11.0526315789473684No Hit
TTTTTTTTTTTGTTTTTTTTTTGTT11.0526315789473684No Hit
TTTGTTTGTTTTGTTGGTGTGTTGG11.0526315789473684No Hit
GTCCTTTCCGTCCTCCCCTTCTAGT11.0526315789473684No Hit
CCTGGTGGTGGTTTTTCGGTGTTGG11.0526315789473684No Hit
TTGTGGTGGGTGGGTGGTGGTGGTG11.0526315789473684No Hit
TTTTTTTTTTTTTTTTTTTTGGTGG11.0526315789473684No Hit
TTTGGTTTTGTTTTGTTTTTTTGGT11.0526315789473684No Hit
TGTTGGGTGGTTTTGGTGTTTTTGG11.0526315789473684No Hit
CCGGTTTGGTGGGGGGGTGTGTGTG11.0526315789473684No Hit
TTTTTTTTTGTTTTTTTTTTTTTTT11.0526315789473684No Hit
TTTTTTTTTTTTTTTTTTTTTGGTC11.0526315789473684No Hit
TTTTAATTATTTTAATTTTTTTTTT11.0526315789473684No Hit
TTGTTTGTGTGTTGGTTGTGGGGGG11.0526315789473684No Hit
TTTGTTTTGTTGTTTGGTTTTTTTT11.0526315789473684No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTTTTG50.019.018
TTTTTGT50.019.019
Produced by FastQC (version 0.11.5)