FastQCFastQC Report
Wed 28 Aug 2019
H2HY7BGXC_n02_Lu_RNAseq_Aug2019_Sample_7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HY7BGXC_n02_Lu_RNAseq_Aug2019_Sample_7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48318
Sequences flagged as poor quality0
Sequence length25
%GC29

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT40098.297114946810712No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG6361.3162796473363962No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT6091.2603998509872099No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA5621.1631276128978847No Hit
TTTTTTTTTTTTTTTTTTTTTTGGG3140.6498613353201705No Hit
TTTTTTTTTTTTTTTTTTTTTGGGG2680.5546587193178526No Hit
TTTTTTTTTTTTTTTTTTTTTTGTT2560.5298232542737696No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG2290.473943457924583No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA2070.4284117720104309No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC1970.4077155511403618No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG1910.39529781861832025No Hit
TTTTTTTTTTTTTTTTTTTTGGGGG1650.3414876443561406No Hit
CGGGGGGGGGGGGGGGGGGGGGGGG1160.24007616209280186No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT1140.23593691791878804No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA840.17384825530858064No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC830.17177863322157375No Hit
TGTTTTTTTTTTTTTTTTTTTTTTG790.1635001448735461No Hit
TTTTTTTTTTTTTTTGTTTTTTGGG710.1469431681774908No Hit
TTTTTTTTTTTTTTTTGGTTTTGGG680.14073430191647004No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA680.14073430191647004No Hit
TTTTTTTTTTTTTTTTTTTTTTGGT680.14073430191647004No Hit
TTTTTTTTTTTTTTTTTTTTTGGTT650.1345254356554493No Hit
TTTTTTTTTTTTTTTGTTTTTTTTT630.1303861914814355No Hit
TTTTTTTTTTTTTTTTTTTTTTTCT600.12417732522041476No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC600.12417732522041476No Hit
TTTTTTTTTTTTTTTTTTTTGGTGG580.12003808104640093No Hit
TTTTTTTTTTTTGTTTTTTTTTTGT570.11796845895939402No Hit
TTTTTTTTTTTTTTTTTTTTTTATT550.11382921478538019No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC540.11175959269837327No Hit
TTTTTTTTTTTTTTTTTTTTTGTTT510.10555072643735255No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTTGGG7100.08.69718319
TTTGGGG3550.08.02816919
CCCCCCC1904.4786415E-46.510
GTTTGGG2051.5802104E-46.487805419
GTTTTGG3553.3060132E-86.154929618
TGTTGGG1950.0042833125.846153718
TTGGGGG2608.62081E-55.846153719
GTTGGGG2100.0097262265.428571719
Produced by FastQC (version 0.11.5)