FastQCFastQC Report
Wed 28 Aug 2019
H2HY7BGXC_n02_Lu_RNAseq_Aug2019_Sample_6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HY7BGXC_n02_Lu_RNAseq_Aug2019_Sample_6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15684
Sequences flagged as poor quality0
Sequence length25
%GC35

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT7875.017852588625351No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT1891.2050497322111706No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG1430.9117572047946952No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG1130.7204794695230808No Hit
TTTTTTTTTTTTTTTTTTTTTTGTT870.5547054322876818No Hit
CGGGGGGGGGGGGGGGGGGGGGGGG770.49094618719714356No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA730.46544248916092834No Hit
GTGGGGGGGGGGGGGGGGGGGGGGG670.42718694210660546No Hit
TTTTTTTTTTTTTTTTTTTTTGGGG550.3506758479979597No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG550.3506758479979597No Hit
CTGGGGGGGGGGGGGGGGGGGGGGG530.3379239989798521No Hit
TTTTTTTTTTTTTTTTTTTTTTGGG470.2996684519255292No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC470.2996684519255292No Hit
TTGGGGGGGGGGGGGGGGGGGGGGG460.2932925274164754No Hit
CCCCCCGGGGGGGGGGGGGGGGGGG430.27416475388931394No Hit
CCCCCGGGGGGGGGGGGGGGGGGGG360.22953328232593728No Hit
TTTTTTTTTTTTTTTTTTTTGGGGG320.204029584289722No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA290.18490181076256057No Hit
TGGGGGGGGGGGGGGGGGGGGGGGG260.16577403723539913No Hit
TTTTTTTTTTTGTTTTTTTTTTGTT240.1530221882172915No Hit
TTTTTTTTGTTTTTTTTTTTTTTGT230.1466462637082377No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT230.1466462637082377No Hit
TTTTTTTTTTTTTTTTTTGTTTGTT220.14027033919918389No Hit
TTTTTTTTTTTTTTGGTTTTTTTGT220.14027033919918389No Hit
TTTTTTTTTTTTTTGGTTTTTTTGG210.13389441469013008No Hit
TTTTTTTTTTTTTTTTTTTTTTGGT210.13389441469013008No Hit
TTTTTTTTTTTTTTTGTTTTTTTTT200.12751849018107625No Hit
TTGTTTGTGTTTGGTTTTTTTTTGT200.12751849018107625No Hit
TTTTTTTTTTTTTTTTTTTTTTATT190.12114256567202243No Hit
TTTTTTTTTTTTTTTTTTTTTGTTT190.12114256567202243No Hit
CCCCGGGGGGGGGGGGGGGGGGGGG190.12114256567202243No Hit
TTTTTTGTTTTGGTTGTTTGTTGTT190.12114256567202243No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA180.11476664116296864No Hit
TGTTTGTTTGTTGGTTTTTTTGTTT180.11476664116296864No Hit
TTTTTTTTTTTTTGTTTTTTTTTTT180.11476664116296864No Hit
TTTTTTTTTTTTTTTTGTTTTTTGT180.11476664116296864No Hit
CGCGGGGGGGGGGGGGGGGGGGGGG170.10839071665391482No Hit
TTTTTGTTGTTGTGTGTTTTTTGGG170.10839071665391482No Hit
TTTTTTTTTTTTGTTTTTTTTTTGT170.10839071665391482No Hit
TTTTTGTTGTTTTTTTTTTTTTTTG160.102014792144861No Hit
TTGTGTGTTGTTGTTGGTTTTTTTG160.102014792144861No Hit
TTTTTTTTGTTTTTTTGTTTTTTGG160.102014792144861No Hit
TTTTTTTTTTTTTTTTTTTTTGGTT160.102014792144861No Hit
TTTGTTTGTTTTTGTTTTTGTTGGT160.102014792144861No Hit
TGTGGTGTTGTTTGTGTTGTGTTGT160.102014792144861No Hit
TTGTTGTGTGTGTTTTTTTTTGTTT160.102014792144861No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTTGGG2250.011.82222219
TTTGGGG855.8517157E-410.05882419
GTTTTGG850.0067732958.94117618
TTTTTGG3903.250534E-96.089743618
Produced by FastQC (version 0.11.5)