FastQCFastQC Report
Wed 28 Aug 2019
H2HY7BGXC_n02_Lu_RNAseq_Aug2019_Sample_27.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HY7BGXC_n02_Lu_RNAseq_Aug2019_Sample_27.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5378
Sequences flagged as poor quality0
Sequence length25
%GC25

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT55510.319821494979546No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT1011.878021569356638No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG881.6362960208255857No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA801.4875418371141689No Hit
TTTTTTTTTTTTTTTTTTTTTTGTT390.7251766455931573No Hit
TTTTTTTTTTTTTTTTTTTTTTGGG380.7065823726292302No Hit
TTTTTTTTTTTTTTTTTTTTTGGGG370.687988099665303No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC340.6322052807735217No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA250.46485682409817775No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG250.46485682409817775No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG170.31610264038676084No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT170.31610264038676084No Hit
TTTTTTTTTTTTTTTTTTTTGGGGG160.29750836742283376No Hit
GTGGGGGGGGGGGGGGGGGGGGGGG150.27891409445890664No Hit
TTTTTTTTTTTTTTTTTTTTTGTTT120.2231312755671253No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA110.2045370026031982No Hit
TTTTTTTTTTTTTTGGTTTTTTTGG110.2045370026031982No Hit
TTTTTTTTTTTTTTTTGTTTTTTTT110.2045370026031982No Hit
TTTTTTTTTTTTTTTGTTTTTTGGG100.1859427296392711No Hit
TTTTTTTTTTTTTTTTTTTTTTATT100.1859427296392711No Hit
TTTTTTTTTTTTTTTGTTTTTTTTT100.1859427296392711No Hit
CGGGGGGGGGGGGGGGGGGGGGGGG100.1859427296392711No Hit
CCCCCCGGGGGGGGGGGGGGGGGGG90.167348456675344No Hit
TTTTTTTTTTTTTTTTTTTTTTGGT90.167348456675344No Hit
TTTTTTTTTTTTTTTTTTTGTTTTT80.14875418371141688No Hit
TTTTTTTTTTTTTTTTTTTTTTACC80.14875418371141688No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC80.14875418371141688No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC80.14875418371141688No Hit
TTTTTTTTTTTTTTTTGGTTTTTTT80.14875418371141688No Hit
TTTTTTTTGTTTTTTTGTTTTTGGG80.14875418371141688No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT70.13015991074748978No Hit
TTTTTTTTTTTGTTTTTTTTTTTTT70.13015991074748978No Hit
TTTTTTTTTTTTTTTTTTGTTTTTT70.13015991074748978No Hit
TTTTTTTTTTTTTTTTGTTTTTTGT70.13015991074748978No Hit
TTTTTTTTTTTTTTTTGGTTTTGGG70.13015991074748978No Hit
TTTTTTTTTTTTTTTTTTTTGGTGG70.13015991074748978No Hit
TTTTTTTTTTTTTTTGTTTTTTTGG70.13015991074748978No Hit
TTTTTTTTTTGTTTTTTTTTTTTTT70.13015991074748978No Hit
TTTTTTTTTTTTTTTTTTTTTTTCT70.13015991074748978No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA70.13015991074748978No Hit
TTTTTTTTTTTTTTTTTTTTGTTTT60.11156563778356265No Hit
TTTGTGTTTTTGTGTGTGTTTTTGG60.11156563778356265No Hit
TTTTTTTTTTTTTTTTTTTTGGTTT60.11156563778356265No Hit
CCCCCGGGGGGGGGGGGGGGGGGGG60.11156563778356265No Hit
TTTTTTTTTTTTGTTTTTTTTTTGT60.11156563778356265No Hit
TTTGTGTTTGTTTTTTGTTTTTTTT60.11156563778356265No Hit
TGGTTGTGGTTGGGTGTTGTTTTTT60.11156563778356265No Hit
TTTTTTTTGTTTTTGTTTTTTGGGG60.11156563778356265No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)