FastQCFastQC Report
Wed 28 Aug 2019
H2HY7BGXC_n02_Lu_RNAseq_Aug2019_Sample_26.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HY7BGXC_n02_Lu_RNAseq_Aug2019_Sample_26.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1205
Sequences flagged as poor quality0
Sequence length25
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT635.228215767634855No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT221.8257261410788383No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG120.9958506224066389No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG110.9128630705394192No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA100.8298755186721992No Hit
CGGGGGGGGGGGGGGGGGGGGGGGG70.5809128630705395No Hit
TTTTTTTTTTTTTTTTTTTTTGTTT60.49792531120331945No Hit
TTTTTTTTTTTTTTTTTTTTTTGTT50.4149377593360996No Hit
CTGGGGGGGGGGGGGGGGGGGGGGG50.4149377593360996No Hit
GTGGGGGGGGGGGGGGGGGGGGGGG50.4149377593360996No Hit
TTTTTTTTTTTTTTTTTTTTTTGGG50.4149377593360996No Hit
TTGGGGGGGGGGGGGGGGGGGGGGG40.33195020746887965No Hit
TTGTGTTGGTGTTGGTGTGTTGTGT40.33195020746887965No Hit
CCCCCCGGGGGGGGGGGGGGGGGGG40.33195020746887965No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA40.33195020746887965No Hit
TTTTTTTTTTTTTTTTTGTTTTTGT30.24896265560165973No Hit
CCCCCGGGGGGGGGGGGGGGGGGGG30.24896265560165973No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC30.24896265560165973No Hit
TTTTGTTTTTGTTTTTTTTTTGTTT30.24896265560165973No Hit
TTTTTTTTTTTTTTTTTTTTGGTGT30.24896265560165973No Hit
TTGTTGTGTGTGTTTTTTTTTGTTT30.24896265560165973No Hit
TTTTTTTTTTTTTTTTTTTTGGTGG30.24896265560165973No Hit
TTTTTTTTTTTGTGTGTTTTTTGTT30.24896265560165973No Hit
TTTTTTTTTTTGTTTTTTTTTTGTT30.24896265560165973No Hit
TGTTTTTTTTTTTTTTTGTTTTTGG30.24896265560165973No Hit
TTGTGTGTTGTTGTTGGTTTTTTTG30.24896265560165973No Hit
TTTTTTTTTTTTTTTTGGTTTTGGG30.24896265560165973No Hit
CGTGGGGGGGGGGGGGGGGGGGGGG20.16597510373443983No Hit
TTTGGTTGGTGTGGTGGGTTGTTTT20.16597510373443983No Hit
GGTGGGGGGGGTGGGGGGGGTGGGG20.16597510373443983No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG20.16597510373443983No Hit
TTGTTGTTTTGTGTGTTGGGTTGTT20.16597510373443983No Hit
TTTGGTTTTTTTTTTTTTTTTTTGT20.16597510373443983No Hit
TTTTTTTGTTGTGGTTTTTTTTTGT20.16597510373443983No Hit
TTTTTTTTTTTTGGTTTGTTTTTTG20.16597510373443983No Hit
TTGTTTGTGTTTGGTTTTTTTTTGT20.16597510373443983No Hit
TTTTTTTTTGTGTTTTTTGTGTGGT20.16597510373443983No Hit
CTCCCCGGGGGGGGGGGGGGGGGGG20.16597510373443983No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC20.16597510373443983No Hit
TTGGTGGTGGTGTGTTTGTTTTTGT20.16597510373443983No Hit
GTTTTTTTTGGTTTTTTTTTTTTTG20.16597510373443983No Hit
TTTTTTTGTTTTTGTTGTGTTTGTT20.16597510373443983No Hit
TTTTTTTTGTTTTTTTTTTGTTTGT20.16597510373443983No Hit
TTTTTTGGGTTTTTTGTTTTTTTGT20.16597510373443983No Hit
TTTTTTTTGTTTTTTTTTTTTTTTT20.16597510373443983No Hit
TTGGTGGTGGTTGTGTGGTTTGTGT20.16597510373443983No Hit
TTTGTTGTTTTGTTGTTTTTTGTGT20.16597510373443983No Hit
TTTTTTTTGGTTTGGTTTTGTTTGG20.16597510373443983No Hit
TTTTGGTGTGGTGGGTGTTTGTGGG20.16597510373443983No Hit
CCCGGGGGGGGGGGGGGGGGGGGGG20.16597510373443983No Hit
TTTTTTTTGTTTGTTGTTTTTTGGG20.16597510373443983No Hit
TTTTTGTTGTTTTTTTTTTTTTTTG20.16597510373443983No Hit
TTTGTTGTTTTTTTTTTTTTTTTGT20.16597510373443983No Hit
TTTTTTTTGTTGTTTGTTGTTTTTG20.16597510373443983No Hit
TTTTTTTTTTTTTTTGTGTTTTTTT20.16597510373443983No Hit
TTGTTGTTTGTTGTTTGTTTGTTTG20.16597510373443983No Hit
TTTTTTTTTTTTTTTTTTGTTTTGT20.16597510373443983No Hit
TTTTTTGTTTGGTTTTTGTTTTGTT20.16597510373443983No Hit
TTTTTTTTTTTTTTGGTTTTTTTGG20.16597510373443983No Hit
TTTTTTTTTTTTTTTTTTTTTTGGT20.16597510373443983No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC20.16597510373443983No Hit
TTGGTGTGTGTGTGTGTTTTGTTGT20.16597510373443983No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)