FastQCFastQC Report
Wed 28 Aug 2019
H2HY7BGXC_n02_Lu_RNAseq_Aug2019_Sample_23.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HY7BGXC_n02_Lu_RNAseq_Aug2019_Sample_23.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATTCCTTGGAGGGTGGTTTTCGGA11.282051282051282No Hit
TCTAGAGCACACCGTACGCCGCCGG11.282051282051282No Hit
TGGTTTTTTTCTGTTTTTTTGGTGG11.282051282051282No Hit
CCTGCTGCGGCCCTGGGGGCTGCCC11.282051282051282No Hit
GGCGTGGGGGGCGTTCCGGGGGCGT11.282051282051282No Hit
TCTTTTTTTCCCTTTCTAATCAAAT11.282051282051282No Hit
CATGCTGCTGTGTTGTTGCCCTGGT11.282051282051282No Hit
CATCCCGTTCCCTCTTCGTGGGTGA11.282051282051282No Hit
CCCACTCTCCGGGCCTCCCCTCCCT11.282051282051282No Hit
CCCAAGTTTCTTTTATGACATGCAT11.282051282051282No Hit
CCGCTTCATAGGCCTTTTCTTACTC11.282051282051282No Hit
TCCCGCGATCCGGGCTTCTTACCCA11.282051282051282No Hit
CCGCTGCGGCGGCGTTCTTCGCATG11.282051282051282No Hit
GAACGTGAGCTTTTTTTAGATCTTC11.282051282051282No Hit
CATGAGCTACCGGTCCCTTCCCTGG11.282051282051282No Hit
CCTTGCGCTTGGTTTCGGCCGTCGT11.282051282051282No Hit
CCATTCTTGTCGCCTTCTTCTTCCT11.282051282051282No Hit
CCCCCTGGGCGGGCTTCTGCCTTCG11.282051282051282No Hit
TATTTTAATAAATTAAAAATTCATT11.282051282051282No Hit
TTCGCCGGTTGCCACCCACTTATTC11.282051282051282No Hit
ACTTGCGCTGGTTTAGCCCTTCGTT11.282051282051282No Hit
TTTTTGTTGTTTTTTGTGTTTTTGT11.282051282051282No Hit
TCTAGTAGCTGTTTCCCTCCGCAGT11.282051282051282No Hit
CCTCCACCTCCCCTTGCCATCGATT11.282051282051282No Hit
GCCCCGGGGCCCTGGGGGGGCCACC11.282051282051282No Hit
CCTCCTTTTCTGTCTTCTGACTTAT11.282051282051282No Hit
TTTGTGGTTGTTGGGTGTTGGGGGG11.282051282051282No Hit
GGCTTGTTGGCCCTTTCGCTTTTGA11.282051282051282No Hit
GAGATATTGGTCCAATTGGGAGTGA11.282051282051282No Hit
ACGTGAGCTTTGATTCGCCCTTCGT11.282051282051282No Hit
CCCTTCCCCCGCCGGGCCCCCGATC11.282051282051282No Hit
TCTCACTTTCCCTACTATTGGGTGA11.282051282051282No Hit
ACTAAAATGTCACGTGAGCTGCCTT11.282051282051282No Hit
TTTTTTTTTTTTTTTTTTTTTTTTT11.282051282051282No Hit
TCAAACGAATTCTCATCTTCCGATG11.282051282051282No Hit
TGTGTTTTTGTTGTTTGCTTTTTTT11.282051282051282No Hit
TGTTTTTTTTTTTTTTTTTTACTTC11.282051282051282No Hit
TTTTTTCTTTTATTTTTTTTTTTTA11.282051282051282No Hit
CGGCCCTCGGGGGTGTGCCGCAGGC11.282051282051282No Hit
TCTTGCTTTGGCTGCAATTTTAGTG11.282051282051282No Hit
TCTTTCCCACCCCCCACTAAAAAAA11.282051282051282No Hit
GCTCCTGCCGGCCCTCCATGCTGCC11.282051282051282No Hit
GTGCCTTCCCTGCGGGCTGTGCTTT11.282051282051282No Hit
CCCCCTCTTTTCCTCCCCTTCTTTT11.282051282051282No Hit
TACCCTACTAAAATTACAAAATTAT11.282051282051282No Hit
CCGGCGCCATCGATTTTCCGGGATA11.282051282051282No Hit
CCATAATTATGGAACTTTATGAATG11.282051282051282No Hit
CTCCGGCCCGCCGGCGGGCGGGGGG11.282051282051282No Hit
AGATGTCTTTTTTCCGAAACGATCT11.282051282051282No Hit
CCTTAGAGATTTGCGAGCGCCGTTC11.282051282051282No Hit
CCTTCTCCCCCGGGGTTTTCCTGTC11.282051282051282No Hit
CCGCCCGGGGGCTGGTGGGGGGGGG11.282051282051282No Hit
AAGCACATCAACCACCCAAAAAACA11.282051282051282No Hit
CCTCCTGTGCTGGCTTCTGCCTTAG11.282051282051282No Hit
TCTTCGTTGGTGGCGCGCTTTGTAT11.282051282051282No Hit
TCTACACACTATTGGTCTCATACCT11.282051282051282No Hit
CCATACCCAACCCAATTTTCTTTAA11.282051282051282No Hit
CTTTCTTCAGTTTACCCATGAATGC11.282051282051282No Hit
TTTATCCCTGTTGTGACTTTTATTA11.282051282051282No Hit
CGTCCGCCCGGTTTAGATGTGCGAA11.282051282051282No Hit
GCTCCCCCCACCCCGCCGCGCCGTG11.282051282051282No Hit
CCCCTCACGCTCGGACTGGGCGGGA11.282051282051282No Hit
TCATTTTCTTATTGTAGTTTCAAAT11.282051282051282No Hit
GGTCTTTCCCTGCCACTACCGCCAG11.282051282051282No Hit
TCCAGTGACCATGCCTCTGGCCTGA11.282051282051282No Hit
TCTCCTTTTCAATTTGTTTTCTTTC11.282051282051282No Hit
CCGCGCGCGTTGCGATCTCATGCCA11.282051282051282No Hit
CCCATTTTCTCCCCTTTTCCTTTTA11.282051282051282No Hit
TTGTTGTTAATTATCTTCTTACATT11.282051282051282No Hit
CCTTTTCCCAAAAAACTATTGAAAT11.282051282051282No Hit
CCTGGGTGGCCCGGCCTGCGTGTTT11.282051282051282No Hit
GGTGCGTGCGCCCCTCGCCGGGGGT11.282051282051282No Hit
CCCCCTCCATGCTGCCACTCGGAAT11.282051282051282No Hit
CATATTTTAGCTTCGCCCCATTTTC11.282051282051282No Hit
TTTTTTTGTGGGTTTGTTTTGTTGG11.282051282051282No Hit
CCCTGATTCCCCGTCACCCTTTTTC11.282051282051282No Hit
GCCTTCCCCCGGCGTTGGGTTCCTC11.282051282051282No Hit
CCTCCGCGCTGCTGCCGTATTGTTC11.282051282051282No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAAAA50.019.01
GTCACGT50.019.09
TAAAATG50.019.03
CACGTGA50.019.011
GAGCTGC50.019.016
TGTCACG50.019.08
GTGAGCT50.019.014
TCACGTG50.019.010
GCTGCCT50.019.018
ATGTCAC50.019.07
CTAAAAT50.019.02
CTGCCTT50.019.019
AAAATGT50.019.04
CGTGAGC50.019.013
AATGTCA50.019.06
TGAGCTG50.019.015
AGCTGCC50.019.017
AAATGTC50.019.05
ACGTGAG50.019.012
Produced by FastQC (version 0.11.5)