FastQCFastQC Report
Wed 28 Aug 2019
H2HY7BGXC_n02_Lu_RNAseq_Aug2019_Sample_22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HY7BGXC_n02_Lu_RNAseq_Aug2019_Sample_22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61
Sequences flagged as poor quality0
Sequence length25
%GC18

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT1321.311475409836063No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA34.918032786885246No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG23.278688524590164No Hit
TATTATAATTATTATTCAACTAATT11.639344262295082No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA11.639344262295082No Hit
TTTTTTTTTTTTTTTTTTTTGGTGT11.639344262295082No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA11.639344262295082No Hit
CGCCCCCCGGGGGGGGGGGGGGGGG11.639344262295082No Hit
TGGTTTTTTTTTATTTTTGATTTTA11.639344262295082No Hit
TGTGTGTGGGTCGTGTGGGTTTTTG11.639344262295082No Hit
TTTTTTGTTTTGGTTTGGGGTGTTG11.639344262295082No Hit
TTTTTTTTTCTTTTTTTTTTTTTTG11.639344262295082No Hit
TTTTTTTTTTTTGTGTGTGTGTTGG11.639344262295082No Hit
TTTATTTTTTTTTTTTTTTTTTTAT11.639344262295082No Hit
TGGTTGTGTGTGGTGTTGTTTGTGG11.639344262295082No Hit
TGTGTGTGGGTGGGGTGGTTTTTGG11.639344262295082No Hit
TTTTTTTTTTTTTTTTTTTTTCCCC11.639344262295082No Hit
TGTTGTTGGTGTTTTTGTTTTTGTT11.639344262295082No Hit
NTTATTTTTTTTTTTTTTTTTTTTT11.639344262295082No Hit
GGGCGGGGGGTGGGGGGGGGGTTGT11.639344262295082No Hit
TTTTTTTTTTTTTTTTTTTTTTCGT11.639344262295082No Hit
TGTGGTGTTTTTGTTGTTTTTTGTT11.639344262295082No Hit
TTTGTGGTGTGGGTTTGTTTTTTTT11.639344262295082No Hit
TGTGTTGTGGTTGGTTTTGTTGGTT11.639344262295082No Hit
TTTTTTTTTTTTTTTTTTTTTTTAG11.639344262295082No Hit
ATCGCCTCGGTTGGCCCCGTTGGTT11.639344262295082No Hit
GGTGGGGGGGGGGGGGGGGGGGGTG11.639344262295082No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT11.639344262295082No Hit
TTTTTTTGTTTGTGTTTTGTTGGGT11.639344262295082No Hit
TTGTTGTGTTGTTTTGTTTGTTTGG11.639344262295082No Hit
TTTTTTTTTTTTTTTTTTTTGGGGG11.639344262295082No Hit
TTTTTTTTTTTTTTTTTTTTTTTCT11.639344262295082No Hit
TTTTTTTTTTTTTTTTTTGGTTTTG11.639344262295082No Hit
TTTTTTTTTTTTTTTTGTTTTGGGG11.639344262295082No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT11.639344262295082No Hit
TTTTTTGTTATCGGTCTTGTTGTAT11.639344262295082No Hit
TTGTGTGGGGTGGGTGGGGGGGGGG11.639344262295082No Hit
TTTTTTTTTTTTTTTGGTTTTTGGG11.639344262295082No Hit
TTTTGTTTTGGTTGTTTTTTTTGGG11.639344262295082No Hit
TTTGTTGTGTTGTTGTGGGTGTGTG11.639344262295082No Hit
TTTTTTTTTTTTTTTTTTTTGTTTT11.639344262295082No Hit
TTTTTTTTTTTTTTCTTTTTTTTTT11.639344262295082No Hit
TTTCTTTTTTTTTTTTTTTTTAAAA11.639344262295082No Hit
TTTTTGTGTGGTGGTGTGTGTTTGG11.639344262295082No Hit
TTTTGTTTTTTTTGTTGTTTTTTTT11.639344262295082No Hit
TTTTTTTTTTTTTTTTTTTTTCCAT11.639344262295082No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTTTTA50.019.019
Produced by FastQC (version 0.11.5)