FastQCFastQC Report
Wed 28 Aug 2019
H2HY7BGXC_n02_Lu_RNAseq_Aug2019_Sample_21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HY7BGXC_n02_Lu_RNAseq_Aug2019_Sample_21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50
Sequences flagged as poor quality0
Sequence length25
%GC36

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTTTTTTGTGGTTTTTGTGGGGG24.0No Hit
TGTTTTGTTTTGGGGGGGTTTTTTG12.0No Hit
TTTTTTTTTTTTTTTTTTATAAAAG12.0No Hit
CTTTTTTGTTGTGGTGTTTTGGTGT12.0No Hit
TTTTGTTTTTTTGTTTGGTGTTGGG12.0No Hit
TTTTTTTTGTTTTTTTGTTTTTGGG12.0No Hit
GTGGGGGGGGGGGGGGGGGGGGGGG12.0No Hit
TTGTTTTTTTTTGTGTTTTTTTTGT12.0No Hit
TTTGTGTTGGTGTGGGGTTTGGGGG12.0No Hit
TGTTTTTTTGTGGTTTTTGTGTAAC12.0No Hit
GGGGTTTTTGGGGTTTTTGTGGGGG12.0No Hit
TGTTTTTTTGTGGTTTTTGTGGGTT12.0No Hit
GGGCGGGGGGGGGGGGGGGGGGGGG12.0No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA12.0No Hit
TTAATTTTTTTTTTTTATTTTTTTC12.0No Hit
TCGTGTGTTGGTGTTGTGGGGGTTG12.0No Hit
TGGTGGGGGGGGGGGGGGGGGGGGG12.0No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG12.0No Hit
GGGTGGGGGGGGGGGGGGGGGTTGG12.0No Hit
GTGTTGTTTTTTTTTTGTGGTGGGG12.0No Hit
GGGGGTTTTGGGGTTTTTGGGGGGG12.0No Hit
TAATTTTTAATTTTATTTTTTTTTT12.0No Hit
TTTTTTTTTTTTTTTTTTTTTTTTT12.0No Hit
CCCCCCGGGGGGGGGGGGGGGGGGG12.0No Hit
TTTTTTTTTTTTTTTTTTTTGGGGT12.0No Hit
TTGTTGTGGGTTGTGGGGTTGGGGT12.0No Hit
TGTGGGGGGGGGGGGGGGTGGTTGG12.0No Hit
TTTTTTGTTGTTTGTTTTTTTTGGG12.0No Hit
TTGGGTTGGTGGTTTGTGGGGGGGG12.0No Hit
TTTTTTTTTTTTTTTTTTTTTTCAA12.0No Hit
TTTGGTGGTTTGTGTTGGGGTGGGG12.0No Hit
TAATATTTATTTAAAAATAATGAAT12.0No Hit
TGTTTGGTTGGGGGGTTTTTTTGGG12.0No Hit
TGTTTTTTGTTTTTTTGTTTTTGGG12.0No Hit
GGGTTTTTTGGGGTTTTTGGGTGGG12.0No Hit
TTTCTTTTTTTTTTTTTTTTTTTGT12.0No Hit
TTTTTTTTTTTTTTTTTGGTTGGGG12.0No Hit
TGTGGTGGTGTTGGTTGTTGTTTTG12.0No Hit
GGGTGTTTTGGGGTTTTTGGGGGGG12.0No Hit
TGTTTGGTGGGTGGGTGGTGTTTTG12.0No Hit
TTTTTTTTGTTTTGTTTTTTTTTGT12.0No Hit
TCTTTTTTTGATGTTTTTGTGTTGT12.0No Hit
TTTTTTTTTTTTTTTTTTTTTTATT12.0No Hit
TTTTTTTTTTTTTTTTTTTTTTGTT12.0No Hit
TTTTTTTTTTTTTTGGTTTTTTTGT12.0No Hit
TATTTTTTTTTTTTTTTTAATTACA12.0No Hit
TGGTTTTTTGGGGTTTTTGGGGGGG12.0No Hit
TTTTTTTTTTTTTATTTTTTTTTTT12.0No Hit
TCGGTGGTGTGTTGTTTTGTTTTGG12.0No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATTTTT50.019.03
TTATTTT50.019.015
TTTTTTC50.019.019
TTTTTTA50.019.011
TTTTTAT50.019.012
TTTTATT50.019.013
TAATTTT50.019.02
TATTTTT50.019.016
TTAATTT50.019.01
TTTATTT50.019.014
ATTTTTT100.09.54
Produced by FastQC (version 0.11.5)