FastQCFastQC Report
Wed 28 Aug 2019
H2HY7BGXC_n02_Lu_RNAseq_Aug2019_Sample_19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HY7BGXC_n02_Lu_RNAseq_Aug2019_Sample_19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40133
Sequences flagged as poor quality0
Sequence length25
%GC27

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT38819.670346099220094No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG5621.4003438566765505No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT5581.390376996486682No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA4831.203498367926644No Hit
TTTTTTTTTTTTTTTTTTTTTTGGG3020.7524979443350858No Hit
TTTTTTTTTTTTTTTTTTTTTTGTT2420.6029950414870555No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG2140.5332270201579747No Hit
TTTTTTTTTTTTTTTTTTTTTGGGG2100.523260159968106No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA1980.4933595793985No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC1630.4061495527371489No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG1470.36628211197767424No Hit
TTTTTTTTTTTTTTTTTTTTGGGGG1370.3413649615030025No Hit
CGGGGGGGGGGGGGGGGGGGGGGGG1150.2865472304587247No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT1050.261630079984053No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA800.1993372037973737No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC710.17691176837016917No Hit
TTTTTTTTTTTTTTTTTTTTTTGGT710.17691176837016917No Hit
TTTTTTTTTTTTTTTGTTTTTTGGG640.15946976303789898No Hit
TTTTTTTTTTTTTTTGTTTTTTTTT630.1569780479904318No Hit
TTTTTTTTTTTTTTTTTTTTGGTGG580.14451947275309596No Hit
TTTTTTTTTTTTTTTTGGTTTTGGG560.1395360426581616No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC540.13455261256322726No Hit
TTTTTTTTTTTTTTTTTTTTTTATT530.13206089751576008No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA530.13206089751576008No Hit
TTTTTGTTTTTTTTTTTTTTTTTGT530.13206089751576008No Hit
TTTTTTTTTTTTGTTTTTTTTTTGT520.1295691824682929No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC520.1295691824682929No Hit
TTTTTTTTTTTTTGTTTTTTTTTTT510.12707746742082576No Hit
CCCCCCGGGGGGGGGGGGGGGGGGG510.12707746742082576No Hit
GTGGGGGGGGGGGGGGGGGGGGGGG500.12458575237335857No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT490.1220940373258914No Hit
CTGGGGGGGGGGGGGGGGGGGGGGG490.1220940373258914No Hit
TTGGGGGGGGGGGGGGGGGGGGGGG450.11212717713602273No Hit
TGTTTTTTTTTTTTTTTTTTTTTTG450.11212717713602273No Hit
TTTTTTTTTTTTTTTTTTTTTGTTT430.10714374704108838No Hit
TTTTTTTTTTTTTTTTTGTTTTTGG410.10216031694615403No Hit
TTTTTTTTTTTTTTTTGTTTTTTGT410.10216031694615403No Hit
TTTTTTTTTTTTTTGGTTTTTTTGG410.10216031694615403No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTTGGG5550.09.75611619
TTTGGGG2400.09.49937719
GTTTTGG2654.199137E-87.169340618
Produced by FastQC (version 0.11.5)