FastQCFastQC Report
Wed 28 Aug 2019
H2HY7BGXC_n02_Lu_RNAseq_Aug2019_Sample_18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HY7BGXC_n02_Lu_RNAseq_Aug2019_Sample_18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10762
Sequences flagged as poor quality0
Sequence length25
%GC36

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT5885.463668463110946No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT1201.1150343802267237No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG920.8548596915071548No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG790.7340643003159264No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA640.594685002787586No Hit
CGGGGGGGGGGGGGGGGGGGGGGGG630.5853930496190299No Hit
GTGGGGGGGGGGGGGGGGGGGGGGG540.5017654711020256No Hit
TTTTTTTTTTTTTTTTTTTTTTGTT510.47388961159635756No Hit
CTGGGGGGGGGGGGGGGGGGGGGGG380.35309422040512917No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG350.32521836089946105No Hit
TTTTTTTTTTTTTTTTTTTTTTGGG340.315926407730905No Hit
CCCCCGGGGGGGGGGGGGGGGGGGG300.27875859505668094No Hit
TTGGGGGGGGGGGGGGGGGGGGGGG290.26946664188812486No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC280.2601746887195689No Hit
TTTTTTTTTTTTTTTTTTTTTGGGG270.2508827355510128No Hit
TGGGGGGGGGGGGGGGGGGGGGGGG270.2508827355510128No Hit
CCCCCCGGGGGGGGGGGGGGGGGGG270.2508827355510128No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA240.22300687604534472No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT200.1858390633711206No Hit
CGCGGGGGGGGGGGGGGGGGGGGGG180.16725515703400856No Hit
TTTTTTTTTTTTTTGGTTTTTTTGG170.1579632038654525No Hit
TTTTTTTTTTTTTTTTTTTTTTGGT170.1579632038654525No Hit
TTTTTGTTGTTTTTTTTTTTTTTTG160.1486712506968965No Hit
TTTTTTTTGTTTTTTTTTTTTTTGT160.1486712506968965No Hit
TTTTTTTTTTTTTTTTGTTTTTTGT160.1486712506968965No Hit
TTTTTTGTTTTGGTTGTTTGTTGTT150.13937929752834047No Hit
TTTTTTTTTTTGTTTTTTTTTTGTT140.13008734435978445No Hit
TTTGTTTGTTTTTGTTTTTGTTGGT140.13008734435978445No Hit
TTTTTTTTTTTTTTTTTTTTGGGGG130.12079539119122838No Hit
TTTTTTTTTTTTTTTTTGTTTTTTT130.12079539119122838No Hit
TTTTTTTTTTTTTTTGTTTTTTTTT130.12079539119122838No Hit
TTTTGTTTTTGTTTTTTTTTTGTTT130.12079539119122838No Hit
TTTTTTTTTTTTTTTTTTTTTTATT120.11150343802267236No Hit
CCGGGGGGGGGGGGGGGGGGGGGGG120.11150343802267236No Hit
TTTTTTTTTTTTTTTTTTGTTTGTT120.11150343802267236No Hit
TTTTTTTTTTTTTTTTTGTTTTTGT120.11150343802267236No Hit
TTTTTTTTTTTTTGTTTTTTTTTGT120.11150343802267236No Hit
TTGTGTGTTGTTGTTGGTTTTTTTG120.11150343802267236No Hit
TTTTTTTTTTTTTGTTTTTTTTTTT110.10221148485411632No Hit
TTTTGTTGTTGTGTGTGTTTTTTTT110.10221148485411632No Hit
CTCCCGGGGGGGGGGGGGGGGGGGG110.10221148485411632No Hit
GTTTTTTTTGGTTTTTTTTTTTTTG110.10221148485411632No Hit
TCCCCCGGGGGGGGGGGGGGGGGGG110.10221148485411632No Hit
TTTTGTTGTTTGGTTTTGTTTGTTG110.10221148485411632No Hit
CCCGGGGGGGGGGGGGGGGGGGGGG110.10221148485411632No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTTGGG1552.7819106E-57.967741519
Produced by FastQC (version 0.11.5)