FastQCFastQC Report
Wed 28 Aug 2019
H2HY7BGXC_n02_Lu_RNAseq_Aug2019_Sample_10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HY7BGXC_n02_Lu_RNAseq_Aug2019_Sample_10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84
Sequences flagged as poor quality0
Sequence length25
%GC13

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT1619.047619047619047No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA44.761904761904762No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA33.571428571428571No Hit
TTTTTTTTTTTTATTTTTTTTTTTT22.380952380952381No Hit
TTTTTTTTTTGTTTTTTTTTTGTTT11.1904761904761905No Hit
TTTTTGTTTTTTTTTTGTGTTGGGT11.1904761904761905No Hit
TTTTTTTGTTTTGTTTTTTTTTTAA11.1904761904761905No Hit
TTGGTTGTTTTTGTGTTTTTTTTGG11.1904761904761905No Hit
TTTTTTTTTTTGGGTTTTTTTGTGT11.1904761904761905No Hit
TTTTTTTGTTTTGTTTGTTGTTGGT11.1904761904761905No Hit
TTTTTTTTTTTGTTTTGTGTTTTTT11.1904761904761905No Hit
TTTGGTTGGTGGTGGGTTTTTTGGG11.1904761904761905No Hit
TTTTTTTTTTGGTTTTTTTTTTTGT11.1904761904761905No Hit
CTTTTTTTTTTTTTTTTTTTTTTTC11.1904761904761905No Hit
TGTGGTGTTGTTGTTTGGTTTTGTT11.1904761904761905No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC11.1904761904761905No Hit
TTTTTTTTTGTTGTTTTTTTTTTTT11.1904761904761905No Hit
TTTTTTTGTTTTTTTTTGGGTTTTT11.1904761904761905No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG11.1904761904761905No Hit
TTTTTTTTTTTTTTTAATTTTTTTT11.1904761904761905No Hit
TTCTGTTTGTTTTTTTGGTTTTTGG11.1904761904761905No Hit
TTTTTTTTTTTTTTGGTTTTTTGTT11.1904761904761905No Hit
TTTTGTTGTGTTTTGTTTTTTTTGT11.1904761904761905No Hit
TTATTTTTTTTTTTTTTTTTTTTTT11.1904761904761905No Hit
TTTTTTTTTTTTTTTTTTTTTGTGT11.1904761904761905No Hit
TTTTTTTTTTTTTTTTTTTTGGGGT11.1904761904761905No Hit
TTTTTTTTTATTTTTTTTTTTTTTA11.1904761904761905No Hit
TTTTTTTTTTTTTTTTTTTGGGTTA11.1904761904761905No Hit
TTTGTGTTTTTGGGTGGTTTTGTTT11.1904761904761905No Hit
TTTGTGTTTTTTTTTTGGTTGTTTT11.1904761904761905No Hit
TTTTTTTTTTTTTTTTTTTTTTACA11.1904761904761905No Hit
TTGTTTTTGTGTTTTTTTTTTTGTG11.1904761904761905No Hit
GTGGGTTTTTTTTTTTTTTTTGGGG11.1904761904761905No Hit
TTTTTTTTTTTGTTTTTTTTTTGGG11.1904761904761905No Hit
TTTTTTTTTTGTTTTTGTGTTTGGG11.1904761904761905No Hit
TTTTTTTTTTTTTTTGTTTTTTGTT11.1904761904761905No Hit
TGTTTGGTTGGTGGGTTTTTTTGTT11.1904761904761905No Hit
TTGTTTTGTTTTTTTTTTTTTTGTT11.1904761904761905No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG11.1904761904761905No Hit
TTTTTTTGGTTTTTTTTGTTTGGTG11.1904761904761905No Hit
GTGGGTGGTGTGTTTGGGGTTGGGG11.1904761904761905No Hit
TTTTTTTTTGTTTTTTTTTTTTTAC11.1904761904761905No Hit
TTTTTTTTTTGTTTTTTTTTTTTGG11.1904761904761905No Hit
TTTTTTTTTTTTTTTTTTTTTGTTT11.1904761904761905No Hit
TTTTTATTTTTTTTTTAAATTTAAA11.1904761904761905No Hit
TTTTTTGTTTTGGTTTTGTGTTTGT11.1904761904761905No Hit
TGGTGTTGGTGTTGTTGTGTTTTGT11.1904761904761905No Hit
TGGTTGTGGTTGGGTGTTGTGGTTT11.1904761904761905No Hit
TTTTCTATTTTTTTTTTTTTTTTTT11.1904761904761905No Hit
TTTTTTTTTTTTTTTGTTTTTTTTT11.1904761904761905No Hit
ATAAAATATTTATTTTAAATTTAAA11.1904761904761905No Hit
ACTTTTTTTGTTTTTTTTCATTTTG11.1904761904761905No Hit
TTGTTGTGGTTGTGTCTGTTGTTGT11.1904761904761905No Hit
TTTTTTTTTGTTGTTGTGTTTTGTT11.1904761904761905No Hit
TTTTTGTGTTGTTGTTGTTTTTGTT11.1904761904761905No Hit
TTTTTTTTTTTTTTGGTTTTTTTGT11.1904761904761905No Hit
GGGTGGGGGGGGGGGGGTTGGTTGG11.1904761904761905No Hit
TTTTTTTTTTTTTTTTTTTGTGTGT11.1904761904761905No Hit
TTTTTTTTTTTTTTTGTTTTTGTTT11.1904761904761905No Hit
TTTGTCTTTGTTATTTTCTTTTTTG11.1904761904761905No Hit
TTTGATGTTTGTGTGTTGTTTTTCG11.1904761904761905No Hit
TTGTGGTGTTGTGTTGTTGTTTTGT11.1904761904761905No Hit
TTGTGGGGGGGGGGTTGTTTGTTGT11.1904761904761905No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTTGTG50.019.018
TTTTTTG50.019.016
TTTTTGT50.019.017
TTTGTGT50.019.019
Produced by FastQC (version 0.11.5)