FastQCFastQC Report
Wed 28 Aug 2019
H2HY7BGXC_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HY7BGXC_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92516867
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1041549011.257936350136024No Hit
CCGAGCCCACGAGACGAACGGTAATCTCGTATGCCGTCTTCTGCTTGAAA2535340.27404084057450845RNA PCR Primer, Index 12 (96% over 28bp)
CCGAGCCCACGAGACGAACGGTACTCTCGTATGCCGTCTTCTGCTTGAAA2124760.22966190586631083Illumina PCR Primer Index 12 (96% over 27bp)
GCCAATAGGCAGCTTTCTTAACTATCCTAACAAGCCTTGGACCAAATGGA1094880.11834382588852689No Hit
TCAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGC1050870.11358685546496079No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACCG69800.038.31822621
CTCTTAT2180100.035.7996941
TCTTATA2117800.035.66852
CGTCTTC2390000.035.26436635
CGGCCTA161550.035.01394310
CTTGAAA1288500.034.7022344
GCCGTCT2423800.034.3766933
ATCCGAC67700.034.30555319
GTCTTCT2464150.034.30533236
CTGCTTG2423200.034.2493841
TGCCGTC2534200.033.8096532
TCTTCTG2511100.033.5143937
TCCGACC81400.032.9655120
ATGCCGT2417650.032.89200631
CCGTCTT2541450.032.78937534
TACTCGG300800.032.7888373
TATACAC1867650.032.4993975
ACCTGCG172000.032.4054875
ACACGTG162000.032.355322
TCTGCTT2567450.032.32206340