FastQCFastQC Report
Wed 28 Aug 2019
H2HY7BGXC_n01_S9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HY7BGXC_n01_S9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9698544
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTATACACATCTCCGAGCCCACGAGACAAGCAACGATCTCGTATGCC487580.5027352559311996No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG227990.23507652282651914No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAAGCAACGCTCTCGTATGCC200520.206752683701801No Hit
TCAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGC199720.20592781761880957No Hit
ACAAAGGTGCTGCTTTTACAGGGAAGCTTATTCTGTTTTAAACATTGAAA188770.19463746310786445No Hit
TCTCTTATACACATCTCCGAGCCCACGAGACAAGCAACGATCTCGTATGC184090.1898119965223646No Hit
GCCCTTGAGAAGGATTATGAGGAGGTTGGTGTGGATTCTGTTGAAGGAGA151110.15580689225104305No Hit
CTGGACCTGCGGCCTAAGAAGACACGTGCCATGCGCCGCCGGCTCAACAA115010.11858481025605493No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC102100.038.35193344
ATACCGC9600.037.35334435
TATACCG9700.036.74145534
CTACTCG102500.036.4293752
GCTACTC207650.035.7843251
TACTCGG101650.035.6731383
CTACTTG113000.035.5173152
GTCGAAT11750.035.01145610
GACACGT21000.034.8876421
GCTACTT118100.034.747431
ACGTGCC21250.034.6842724
TCCGACG8750.034.44335641
AGGCTGA431700.034.4127111
CCGTAAC12550.034.18092741
TACTTGG118550.034.151343
CGTAACC12950.034.14438242
TGCCGTA16100.033.8874539
GAATCGC79750.033.71220825
CGGAACT12100.033.641872
AGAATCG83350.033.3383824