FastQCFastQC Report
Wed 28 Aug 2019
H2HY7BGXC_n01_S7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HY7BGXC_n01_S7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7722012
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTATACACATCTCCGAGCCCACGAGACCAGAACTGATCTCGTATGCC665930.8623788722421049No Hit
TCTCTTATACACATCTCCGAGCCCACGAGACCAGAACTGATCTCGTATGC257240.333125615448409No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAGAACTGCTCTCGTATGCC246900.3197353228666311No Hit
ACAAAGGTGCTGCTTTTACAGGGAAGCTTATTCTGTTTTAAACATTGAAA206080.2668734521521075No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG181900.23556036949955528No Hit
TCAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGC177390.2297199227351628No Hit
GCCCTTGAGAAGGATTATGAGGAGGTTGGTGTGGATTCTGTTGAAGGAGA144260.18681659650360555No Hit
TCTCTTATACACATCTCCGAGCCCACGAGACCAGAACTGCTCTCGTATGC109240.1414657216279902No Hit
CACAAAGGTGCTGCTTTTACAGGGAAGCTTATTCTGTTTTAAACATTGAA107730.13951027271130892No Hit
GCTGGCCCAAGGTGTCCTGCAGGCTGTAATGCAGTTTAATCAGAGTGCCA89550.1159671857541791No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC158150.039.16955644
ATACCGC6050.037.4544635
GTCGAAT7700.037.42703210
CGTACTA5600.036.13973642
CTACTCG41600.036.0735362
TACTCGG41750.035.4689063
TATACCG6600.035.33325634
CGCGTCG250.00235277935.2024326
TCCGACG5750.035.1969641
GGTCGAA8350.034.7774669
GCTACTC87600.034.7146531
TACCGCA6800.033.97051636
GCCGTAA16300.033.8743740
CCGTAAC11900.033.8290141
ACGTGCC13100.033.75805724
TGGTCGA8700.033.631028
CGTAACC12250.033.58077242
TGCCGTA16400.033.2682239
AGGCTGA178700.033.07866711
GAATCGC32700.033.0359225