FastQCFastQC Report
Wed 28 Aug 2019
H2HY7BGXC_n01_S6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HY7BGXC_n01_S6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9197964
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGACCTGCGGCCTAAGAAGACACGTGCCATGCGCCGCCGGCTCAACAA320710.3486749893780841No Hit
TCAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGC261890.28472605459208145No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAACCAGGTATCTCGTATGCC233950.2543497669701686No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG212750.23130118795855256No Hit
GCCAATAGGCAGCTTTCTTAACTATCCTAACAAGCCTTGGACCAAATGGA192090.2088396953934588No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAACCAGGTATCTCGTATGGC169790.18459519954633438No Hit
GCTGGAGCTGGCAAGGTCACCAAGTCTGCCCAGAAAGCTCAGAAGGCTAA167960.1826056288108977No Hit
GCCTTCGGAGCGTTCTCTGTCCTACTTCTGACTTTACTTGTGGTGTGACC162720.17690871588538506No Hit
TCTCTTATACACATCTCCGAGCCCACGAGACAACCAGGTATCTCGTATGC110230.11984173888917155No Hit
CAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGCG104650.11377517894177451No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACACGT49450.039.8196321
ACGTGCC49850.039.23530624
AGACACG51500.038.78993620
CACGTGC50850.038.46371523
CGGCCTA52350.038.3673210
ACACGTG51350.038.30341722
TCGGAGC22350.038.199155
GCGTTCT22500.038.1319910
CGTTCTC23000.037.6856311
ATGCGCC47300.037.57884631
CGGAGCG23000.037.502696
ATACCGC12050.037.42688435
GAGCGTT23150.037.1563578
TATACCG12050.037.0617434
ACCTGCG55850.036.167545
TGCGGCC57800.035.9675948
GCGGCCT59150.035.5569
GCGCCGC46200.035.42809733
CGCCGGC49250.035.24419437
CGCCGCC46350.035.1235834