FastQCFastQC Report
Wed 28 Aug 2019
H2HY7BGXC_n01_S5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HY7BGXC_n01_S5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9221893
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGACCTGCGGCCTAAGAAGACACGTGCCATGCGCCGCCGGCTCAACAA331630.3596116328827498No Hit
TCAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGC315980.34264114753879704No Hit
GCCAATAGGCAGCTTTCTTAACTATCCTAACAAGCCTTGGACCAAATGGA245030.2657046660593438No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTCTACCAATCTCGTATGCC241840.2622455064269342No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG217720.23609035585210109No Hit
GCCTTCGGAGCGTTCTCTGTCCTACTTCTGACTTTACTTGTGGTGTGACC173430.1880633401406848No Hit
GCTGGAGCTGGCAAGGTCACCAAGTCTGCCCAGAAAGCTCAGAAGGCTAA155760.16890241515489282No Hit
CAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGCG133890.1451871107157717No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTCTACCACTCTCGTATGCC109500.11873917860465306No Hit
GCAGGAAGGGGGGTCGCCGTGGTCGCCGTCTGTGAACAAGATTCCTCAAA101990.11059551439167642No Hit
TCTCTTATACACATCTCCGAGCCCACGAGACGTCTACCAATCTCGTATGC95340.1033844135905719No Hit
CCGGAACTGGTCGAATGAGGCACCTAAAAATTGTATACCGCAGATTCAGG95150.10317838213911178No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACACGT55250.040.17856621
AGACACG56450.040.02599320
ACGTGCC54600.039.9718824
CGGCCTA57550.039.4501610
ACACGTG56100.039.41293322
ATACCGC13300.039.0370635
CACGTGC56450.038.89575623
ATGCGCC49850.038.43712631
GCGTTCT24000.038.40759710
ACCTGCG58550.038.2569775
TATACCG13550.038.1544634
CGTTCTC24900.037.9908311
CGGAGCG24650.037.93716
GCGGCCT64200.037.5228549
TCGGAGC24800.037.4412655
GTCGAAT17100.037.30922710
CGGAACT17300.037.137882
GAGCGTT24800.037.080148
TGCGGCC64200.036.73478
GCGCCGC48700.036.59087833