FastQCFastQC Report
Wed 28 Aug 2019
H2HY7BGXC_n01_S4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HY7BGXC_n01_S4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6487837
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGC169480.2612272780589278No Hit
CTGGACCTGCGGCCTAAGAAGACACGTGCCATGCGCCGCCGGCTCAACAA165410.2549540008480484No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCTATCCGATCTCGTATGCC162260.2500987617290632No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG140710.21688276077219573No Hit
GCCTTCGGAGCGTTCTCTGTCCTACTTCTGACTTTACTTGTGGTGTGACC135560.20894483014909285No Hit
GCTGGAGCTGGCAAGGTCACCAAGTCTGCCCAGAAAGCTCAGAAGGCTAA113330.1746807140808254No Hit
GCCAATAGGCAGCTTTCTTAACTATCCTAACAAGCCTTGGACCAAATGGA112430.17329350290397247No Hit
GCCCTTGAGAAGGATTATGAGGAGGTTGGTGTGGATTCTGTTGAAGGAGA68900.10619872231685229No Hit
CCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCA68570.10569007821867288No Hit
CCTTTGGGTCGCTTTGCTGTTCGTGATATGAGACAGACAGTTGCGGTGGG66020.1017596465509229No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACACGT27700.039.0782221
AGACACG28300.038.7161720
ATACCGC7050.038.6941435
CGGCCTA29450.038.39580510
TATACCG7150.038.15296634
TCGGAGC18150.037.9455345
CGGAGCG18100.037.928796
GCGTTCT18500.037.339110
ACGTGCC29050.036.95924824
ACACGTG29350.036.73139622
GAGCGTT18850.036.2956858
ATGCGCC26600.036.1403531
TCCGACG10000.036.07777441
CACGTGC29950.035.84861823
CGTTCTC19450.035.5153411
ACCTGCG31600.035.5120775
GCGGCCT34550.035.4660729
TGCGCGA6000.034.8384364
GTCGAAT9550.034.78410
TGCGGCC34300.034.3135458