FastQCFastQC Report
Wed 28 Aug 2019
H2HY7BGXC_n01_S3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HY7BGXC_n01_S3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7243079
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG179660.24804368418458503No Hit
CCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCA130380.1800063205164544No Hit
GCCTTCGGAGCGTTCTCTGTCCTACTTCTGACTTTACTTGTGGTGTGACC108100.1492459215203921No Hit
TCAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGC94560.1305522140515104No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCCTCAATATCTCGTATGCC89430.12346959076381743No Hit
CCCTCTACACTTATCATCTTCACAATTCTAATTCTACTGACTATCCTAGA75270.10391989373579938No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGACG19400.038.10103241
TTCCGAC19800.036.3313740
GCGTTCT16500.036.13287410
CGGAGCG16800.036.0171666
CGACGGC20500.035.3054143
GAATCGC37900.035.1777925
GAGCGTT17150.035.0202038
AATCGCT39150.034.78516426
TACTTCG21650.034.64939517
AGAATCG40000.034.48599224
CGCTTGA43750.034.34328529
TTTCCGA20900.033.99933639
TTCGAGT22400.033.98311620
TCGGAGC17650.033.9084055
TCGCTTG46300.033.8315728
GACACGT11900.033.64802621
GAACCCG43850.033.61393734
TGCGCGA5400.033.4118274
CTACTCG51700.033.3233262
AGGCTGA236950.033.3129311
Produced by FastQC (version 0.11.5)