FastQCFastQC Report
Wed 28 Aug 2019
H2HY7BGXC_n01_S2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HY7BGXC_n01_S2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8193903
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTATACACATCTCCGAGCCCACGAGACTTCCATCCATCTCGTATGCC226600.2765470862908677No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG197390.24089862913925147No Hit
GCCTTCGGAGCGTTCTCTGTCCTACTTCTGACTTTACTTGTGGTGTGACC190370.23233128339449466No Hit
CTGGACCTGCGGCCTAAGAAGACACGTGCCATGCGCCGCCGGCTCAACAA189700.23151360224791534No Hit
TCAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGC174900.21345139184586392No Hit
GCTGGAGCTGGCAAGGTCACCAAGTCTGCCCAGAAAGCTCAGAAGGCTAA135880.16583061820477982No Hit
GCCAATAGGCAGCTTTCTTAACTATCCTAACAAGCCTTGGACCAAATGGA122440.14942817848832235No Hit
TCTCTTATACACATCTCCGAGCCCACGAGACTTCCATCCATCTCGTATGC91860.11210774645489457No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAGCG27050.039.4513446
CGGCCTA34400.039.3312510
GACACGT33400.039.12703321
TCGGAGC27500.039.125825
AGACACG34200.038.4052420
GCGTTCT27800.037.98546610
GAGCGTT28300.037.8585178
CGTTCTC28200.037.75826311
ATGCGCC30100.037.49258831
ACACGTG35250.036.9487322
ACGTGCC35050.036.7201824
ATACCGC7850.036.1522935
TATACCG7900.035.92347734
CACGTGC35950.035.739723
TCCGACG13300.035.39620241
GTCCTAC29800.035.36055819
GCGGCCT42200.035.189449
CGCCGGC32150.034.89835437
ACCTGCG39250.034.8128245
GCGCCGC30050.034.628433