FastQCFastQC Report
Wed 28 Aug 2019
H2HY7BGXC_n01_S27.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HY7BGXC_n01_S27.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1268983
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGACCTGCGGCCTAAGAAGACACGTGCCATGCGCCGCCGGCTCAACAA47420.3736850690671191No Hit
TCAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGC41550.3274275541910333No Hit
GCCAATAGGCAGCTTTCTTAACTATCCTAACAAGCCTTGGACCAAATGGA34370.2708468119746285No Hit
CCTCTGGGGGTCCTCTCTGAGTCAAATCCAGTGGTGGGTAATTGTACAAT29130.2295539026133526No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28040.2209643470401101No Hit
CAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGCG19740.15555763946404325No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAGAACTGATCTCGTATGCC18910.14901696870643658No Hit
GCTGGAGCTGGCAAGGTCACCAAGTCTGCCCAGAAAGCTCAGAAGGCTAA18340.14452518276446571No Hit
GCCCTTGAGAAGGATTATGAGGAGGTTGGTGTGGATTCTGTTGAAGGAGA17590.13861493810397776No Hit
GCAGGAAGGGGGGTCGCCGTGGTCGCCGTCTGTGAACAAGATTCCTCAAA15110.11907172909329754No Hit
CCTTCCTGGGGGCCTTGGCTTTGATCTACAATGAAGCCCTCAAGGGCTGA14130.11134900940359327No Hit
CCGGAACTGGTCGAATGAGGCACCTAAAAATTGTATACCGCAGATTCAGG13560.10685722346162241No Hit
GGCCGATGGCATCGTCTCAAAGAACTTTTGACTGGAGAGAATCACAGATG13380.10543876474310532No Hit
CTCAAAAAGGACACTTCTGATTAAGACGGTTGAAACTAGAGATGGACAGG13200.1040203060245882No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCAT254.4438766E-543.998739
AGACACG7400.040.4328320
GACACGT7550.039.92092521
ACGTGCC7650.038.53632424
ACCTGCG7950.038.1950765
CACGTGC7850.037.83476623
CGCCTCG1050.037.71465723
ATGCGCC7500.037.54407531
ACACGTG8000.037.40036822
CGGCCTA8450.037.2340710
CGAATGA1950.037.23113612
GCCGTTA301.3001912E-436.6713681
GCGAATG1800.036.671361
CGCCGCC6900.036.66558534
CGCCATA301.3016994E-436.66414343
CGGAACT2050.036.4924772
ACGGTGC850.036.23279629
TGCGCGA553.423338E-936.0031934
TGCGCCG7050.035.8840532
GGCCTAA8700.035.65833311
Produced by FastQC (version 0.11.5)