FastQCFastQC Report
Wed 28 Aug 2019
H2HY7BGXC_n01_S26.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HY7BGXC_n01_S26.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1377326
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATAGCGACGTCGCTTTT48620.3530028475466229No Hit
GCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTG39170.2843916400329334No Hit
CCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCACAGA34640.25150182309779967No Hit
ACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACC32500.23596447028517578No Hit
GCGATGCGGCGGCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAAC27050.19639504372966168No Hit
TCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGATAGCTGGCGCTCTC26680.19370867899103045No Hit
GCCGCATCGCCGGTCGGCATCGTTTATGGTCGGAACTACGACGGTATCTG22900.1662641959855546No Hit
TCAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGC20780.1508720520777216No Hit
GCTTGTGGCGGCCAAGCGTTCATAGCGACGTCGCTTTTTGATCCTTCGAT20540.14912954522023109No Hit
AACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACT17200.12487965812015456No Hit
CCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCACAGG16670.12103162214319632No Hit
CCTCTGGGGGTCCTCTCTGAGTCAAATCCAGTGGTGGGTAATTGTACAAT16200.1176192128806107No Hit
TCTGACCTTTTGGGTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAGGG15660.113698572451257No Hit
GCGACGGCCGGGTATGGGCCCGACGCTCCAGCGCCATCCATTTTCAGGGC15500.11253690121292997No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15430.1120286700461619No Hit
CCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATACTCCCACAGG15420.11195606559376647No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACATCGAGGTC15220.11050397654585768No Hit
GCACTTACTGGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCGATC15060.10934230530753068No Hit
GCTCACGTTCCCTATTAGTGGGTGAACACTCCAACGCTTGGTGAATTCTG14620.10614770940213139No Hit
CTGGACCTGCGGCCTAAGAAGACACGTGCCATGCGCCGCCGGCTCAACAA14610.10607510494973593No Hit
CCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATACTCCCACAGA14480.10513124706859525No Hit
CATAGCGACGTCGCTTTTTGATCCTTCGATGTCGGCTCTTCCTATCATTG14430.10476822480661803No Hit
GCCAATAGGCAGCTTTCTTAACTATCCTAACAAGCCTTGGACCAAATGGA14020.10179144225840506No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGGC1400.040.85552614
TCACGTT11850.040.111333
CTCACGT11900.039.942792
ACGTTCC11850.039.3685235
ATAGCGG2700.039.1095627
GAGGCGT16950.038.4173625
AGGCGTT16950.038.1577826
TTCCGTC5050.037.89948719
CACGTTC12850.037.332324
CCATCGG2800.036.9271119
CGTTATT4250.036.75148813
ATGCGGC4850.036.7514884
CGATGCG4800.036.675872
TACCGTA900.036.66521524
CGTTCCC12800.036.618566
GCTCACG13800.036.0380251
TCCGTCA4900.035.91694320
CCGGTCT1900.035.89331412
GTCATAC7300.035.86159535
CTTCCGT5350.035.7742918
Produced by FastQC (version 0.11.5)