FastQCFastQC Report
Wed 28 Aug 2019
H2HY7BGXC_n01_S25.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HY7BGXC_n01_S25.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1299632
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTATACACATCTCCGAGCCCACGAGACTCTATCCGATCTCGTATGCC135571.0431414431162052No Hit
CTGGACCTGCGGCCTAAGAAGACACGTGCCATGCGCCGCCGGCTCAACAA108830.8373908921910203No Hit
TCTGCTGAGGGGGCAGGCGGAGCTTGAGGAAACCGCAGATAAGTTTTTTT61530.47344171273098845No Hit
TCTCTTATACACATCTCCGAGCCCACGAGACTCTATCCGATCTCGTATGC60260.4636697157349158No Hit
GCCAATAGGCAGCTTTCTTAACTATCCTAACAAGCCTTGGACCAAATGGA52440.40349883659374347No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCTATCCGCTCTCGTATGCC36790.28308013345316213No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27870.21444531990594262No Hit
ATCTGAAAGAGGTTATTCGGGAAAGAAAAGAAAGAGAAGAATGGGCAAAG25470.19597855392911226No Hit
GCTCTAGGAGGGCTGGCAACTTAGAGGTGGGGAGCAGAGAATTCTCTTAT25350.19505521563027073No Hit
TCAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGC22330.17181786844275918No Hit
CCTCTGGGGGTCCTCTCTGAGTCAAATCCAGTGGTGGGTAATTGTACAAT20250.1558133379295062No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCTCTCCGATCTCGTATGCC19120.14711856894874856No Hit
GACGCTGGGGATGCTGGTACAAGTTGTGGGACTGCATGCTACTGTCTAGA17940.13803907567680695No Hit
GCTGGAGCTGGCAAGGTCACCAAGTCTGCCCAGAAAGCTCAGAAGGCTAA17460.1343457224814409No Hit
TCTCTTATACACATCTCCGAGCCCACGAGACTCTATCCGCTCTCGTATGC16920.13019070013665407No Hit
CCTTCCTGGGGGCCTTGGCTTTGATCTACAATGAAGCCCTCAAGGGCTGA16790.12919041697957576No Hit
GCTATTCGCCGTTTGGCTCGGCGCGGTGGCGTCAAGCGCATTTCCGGTCT14920.11480172848929544No Hit
CGGATGCGTGGCCTTCTGGCCAGGCGTCTGCGAAATCATATGGCTGTAGC14900.11464783877282185No Hit
CTCTTATACACATCTCCGAGCCCTCGAGACTCTATCCGATCTCGTATGCC14160.10895391926329914No Hit
GCTGGTGGCAGGGCATGAGGACAGCAATGGTTGTATCAACTATGAAGAGC13850.10656862865795855No Hit
GTGAATGGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13190.10149026801433021No Hit
CCCCAGAGGGAGAGTTTGTGTCCATGGGTGTTATCTCTGATGGCAACTCC13040.1003360951407783No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCCTA17200.042.46422610
GACACGT16300.042.3794821
ATGCGCC15450.042.14798431
AGACACG16650.041.62074720
ACGTGCC16500.041.46579424
ACACGTG16700.041.2326722
ACCTGCG17350.041.2158555
CGGCTCA16550.041.20759640
CACGTGC16700.041.10093323
TGCGGCC18200.040.2519048
GGCCTAA18100.040.10965311
GTATGCC30200.039.91969344
CTCGGCG2550.039.68544417
GCGGCCT18850.039.6808669
CGCCGTT2500.039.599177
GCGCCGC15450.039.5849333
CCTGCGG18150.039.5204056
CGCCGCC15500.039.17337434
CCGGCTC17300.039.03964639
CATGCGC16800.039.0229930
Produced by FastQC (version 0.11.5)