FastQCFastQC Report
Wed 28 Aug 2019
H2HY7BGXC_n01_S24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HY7BGXC_n01_S24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1231454
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTATACACATCTCCGAGCCCACGAGACGCCTCAATATCTCGTATGCC105180.8541122932728302No Hit
CTGGACCTGCGGCCTAAGAAGACACGTGCCATGCGCCGCCGGCTCAACAA96870.7866310881283426No Hit
TCTGCTGAGGGGGCAGGCGGAGCTTGAGGAAACCGCAGATAAGTTTTTTT68090.5529236171225235No Hit
TCTCTTATACACATCTCCGAGCCCACGAGACGCCTCAATATCTCGTATGC45160.36672096562275164No Hit
GCCAATAGGCAGCTTTCTTAACTATCCTAACAAGCCTTGGACCAAATGGA44700.3629855439180026No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCCTCAATCTCTCGTATGCC34870.28316120618390944No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG32150.2610734952340891No Hit
GCTCTAGGAGGGCTGGCAACTTAGAGGTGGGGAGCAGAGAATTCTCTTAT26350.21397469982638412No Hit
ATCTGAAAGAGGTTATTCGGGAAAGAAAAGAAAGAGAAGAATGGGCAAAG23140.1879079527128094No Hit
CCTTCCTGGGGGCCTTGGCTTTGATCTACAATGAAGCCCTCAAGGGCTGA18470.14998530192764No Hit
TCTCTTATACACATCTCCGAGCCCACGAGACGCCTCAATCTCTCGTATGC17990.14608747058355406No Hit
CGGATGCGTGGCCTTCTGGCCAGGCGTCTGCGAAATCATATGGCTGTAGC16070.13049614520721034No Hit
GCTATTCGCCGTTTGGCTCGGCGCGGTGGCGTCAAGCGCATTTCCGGTCT15110.12270048251903846No Hit
CCTCTGGGGGTCCTCTCTGAGTCAAATCCAGTGGTGGGTAATTGTACAAT14590.1184778318962787No Hit
TCAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGC14200.1153108439292089No Hit
GCTGGAGCTGGCAAGGTCACCAAGTCTGCCCAGAAAGCTCAGAAGGCTAA13130.10662192822468398No Hit
GCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGCGGAGCTTGAGGAAACCG12450.10110000048722892No Hit
GCCCTTGAGAAGGATTATGAGGAGGTTGGTGTGGATTCTGTTGAAGGAGA12390.10061277156921819No Hit
GACGCTGGGGATGCTGGTACAAGTTGTGGGACTGCATGCTACTGTCTAGA12390.10061277156921819No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGAT207.8559504E-444.00085425
ATGCGCC12900.042.6312931
CGGCCTA15050.042.53384810
GACACGT14350.042.00778621
AGACACG14600.041.59153720
TTCGCCG2600.041.4774975
ACACGTG14500.041.42149422
ACCTGCG15100.041.247665
TGCGGCC15650.041.184288
CACGTGC14450.041.10806723
ACGTGCC14450.040.80356224
CCGGCTC14850.040.44194439
CGCCGGC13850.040.1852437
TACGCTT1650.039.99590344
GCGGCCT16450.039.983759
GCGCCGC13250.039.84659233
TGCGCCG13350.039.7112932
CGGCTCA15150.039.63950340
GGCCTAA15900.039.56827511
CGCCGTT2350.039.315127
Produced by FastQC (version 0.11.5)