FastQCFastQC Report
Wed 28 Aug 2019
H2HY7BGXC_n01_S23.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HY7BGXC_n01_S23.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1563396
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTATACACATCTCCGAGCCCACGAGACCTCTGGTAATCTCGTATGCC258421.6529401380072613No Hit
TCTCTTATACACATCTCCGAGCCCACGAGACCTCTGGTAATCTCGTATGC107780.6893966723721949No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTCTGGTACTCTCGTATGCC103060.6592059849200075No Hit
TCTGCTGAGGGGGCAGGCGGAGCTTGAGGAAACCGCAGATAAGTTTTTTT95300.6095704479223434No Hit
CTGGACCTGCGGCCTAAGAAGACACGTGCCATGCGCCGCCGGCTCAACAA87030.5566727815601422No Hit
CCTCTGGGGGTCCTCTCTGAGTCAAATCCAGTGGTGGGTAATTGTACAAT58970.3771917031897229No Hit
TCTCTTATACACATCTCCGAGCCCACGAGACCTCTGGTACTCTCGTATGC49880.3190490445159128No Hit
GCCAATAGGCAGCTTTCTTAACTATCCTAACAAGCCTTGGACCAAATGGA45530.2912249999360367No Hit
GCTCTAGGAGGGCTGGCAACTTAGAGGTGGGGAGCAGAGAATTCTCTTAT45460.29077725668992377No Hit
TCAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGC44440.2842529979608493No Hit
ATCTGAAAGAGGTTATTCGGGAAAGAAAAGAAAGAGAAGAATGGGCAAAG38110.24376421584806407No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG36890.23596069070152415No Hit
CCTTCCTGGGGGCCTTGGCTTTGATCTACAATGAAGCCCTCAAGGGCTGA36730.23493727756755167No Hit
CTCTTATACACATCTCCGAGCCCTCGAGACCTCTGGTAATCTCGTATGCC24240.1550470897968269No Hit
GCTATTCGCCGTTTGGCTCGGCGCGGTGGCGTCAAGCGCATTTCCGGTCT21220.1357301668930968No Hit
GCTGGAGCTGGCAAGGTCACCAAGTCTGCCCAGAAAGCTCAGAAGGCTAA20430.13067706454410782No Hit
CAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGCG20270.12965365141013538No Hit
CCCATAGAGGGCTGCGTCACTTCTGGGGCCTTCGTGTCCGAGGCCAGCAC19770.12645548536647144No Hit
CCGGAACTGGTCGAATGAGGCACCTAAAAATTGTATACCGCAGATTCAGG19110.12223390618883508No Hit
GCGAATGCGCAGGCTGAAGCGCAAAAGAAGAAAGATGAGGCAGAGGTCCA16780.10733045242536121No Hit
GCCATTGGCTATTTAAAACTTGTAATTTTTTTAATTTACAAAAATATAAA16310.10432417634431712No Hit
CCCCAGAGGGAGAGTTTGTGTCCATGGGTGTTATCTCTGATGGCAACTCC16270.10406832306082402No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCGCC11400.042.25916731
AGACACG12550.042.07446720
GACACGT12350.041.8650921
CGGCCTA13150.041.8238210
CACGTGC12250.041.66803423
TTCCGGT3500.041.48179242
ACCTGCG13250.041.1879585
GTATGCC53450.040.78566744
TATACCG2700.040.739534
ACGTGCC12600.040.685224
TCCGGTC3700.040.4286143
ACACGTG12750.040.37911222
TTCGCCG3600.040.3437165
CGCCGTT3450.040.1726887
ATACCGC2800.040.07020635
CGGCTCA13200.039.8295740
GCGCGAT1050.039.8057629
CTCGGCG3650.039.7770617
GCGCCGC11850.039.7287433
CCGCCGG12300.039.5272536
Produced by FastQC (version 0.11.5)