FastQCFastQC Report
Wed 28 Aug 2019
H2HY7BGXC_n01_S22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HY7BGXC_n01_S22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7327925
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG216660.29566350638141087No Hit
GCCCTTGAGAAGGATTATGAGGAGGTTGGTGTGGATTCTGTTGAAGGAGA213580.2914604065953186No Hit
TCAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGC150000.204696418153843No Hit
ACAAAGGTGCTGCTTTTACAGGGAAGCTTATTCTGTTTTAAACATTGAAA112500.15352231361538224No Hit
GCTGGAGCTGGCAAGGTCACCAAGTCTGCCCAGAAAGCTCAGAAGGCTAA100240.13679179303827482No Hit
GCCTTCGGAGCGTTCTCTGTCCTACTTCTGACTTTACTTGTGGTGTGACC95130.12981846839316724No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAACGGTAATCTCGTATGCC94220.12857664345636727No Hit
GGCAGTGGAAGGAAAGAAGCATGGTCTACTTTAGGTGTGCGCTGGGTCTC91370.12468741151144423No Hit
CACAAAGGTGCTGCTTTTACAGGGAAGCTTATTCTGTTTTAAACATTGAA88970.12141226882098274No Hit
GGATTATGAGGAGGTTGGTGTGGATTCTGTTGAAGGAGAGGGTGAGGAAG80180.10941705871716755No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTT15450.036.4520878
GCGTTCT14950.036.34659210
CGTTCTC15050.036.1048411
CCTTCGG16000.035.205292
CGGAGCG15750.035.065356
TCGGAGC15950.034.763615
GCACTTT146150.034.3712271
GACACGT11400.033.9663121
ATACCGC5100.033.21523735
GCTACGC4600.033.0049631
TATACCG5400.032.99955434
TACTCGG77750.032.9974333
GCTACTT93450.032.8224831
TACCCTA22650.032.73133517
CTACTTG91050.032.624342
CGCCGGC10400.032.57604237
CTACTCG78200.032.5266272
GCGACGT2300.032.52041213
AGGCCGA71500.032.49098611
GCTACTC157550.032.45681