FastQCFastQC Report
Wed 28 Aug 2019
H2HY7BGXC_n01_S21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HY7BGXC_n01_S21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7097874
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTTGAGAAGGATTATGAGGAGGTTGGTGTGGATTCTGTTGAAGGAGA214130.3016818838993197No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG205630.28970646703505865No Hit
TCAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGC150970.21269749223499884No Hit
ACAAAGGTGCTGCTTTTACAGGGAAGCTTATTCTGTTTTAAACATTGAAA122120.17205151852512457No Hit
CACAAAGGTGCTGCTTTTACAGGGAAGCTTATTCTGTTTTAAACATTGAA98710.1390698116083774No Hit
GGCAGTGGAAGGAAAGAAGCATGGTCTACTTTAGGTGTGCGCTGGGTCTC88910.1252628604001705No Hit
GCTGGCCCAAGGTGTCCTGCAGGCTGTAATGCAGTTTAATCAGAGTGCCA83350.11742952889837154No Hit
GCCTTCGGAGCGTTCTCTGTCCTACTTCTGACTTTACTTGTGGTGTGACC81850.11531622003997254No Hit
CAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGCG80820.11386508129053856No Hit
GGATTATGAGGAGGTTGGTGTGGATTCTGTTGAAGGAGAGGGTGAGGAAG77280.10887767238471689No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG5850.039.1106434
TCGGAGC13300.038.381975
GCGTTCT13650.037.06810810
CGGAGCG13450.036.8087546
GAGCGTT13350.036.4185268
ATACCGC6300.036.31702835
CGTTCTC13900.035.92686511
CGGTACG505.9517333E-835.20081721
GCACTTT135050.034.116931
TACCCTA24550.033.87236417
CCGTAAC5650.033.48491341
TGCCGTA7600.033.28930739
CCTTCGG15300.033.2202032
GTCGAAT7450.033.072510
CGTTACG608.021743E-932.99960734
AGCGTTC15200.032.8544049
TTCGGAG15750.032.5506864
GACACGT10050.032.3987621
GGTCTAC19350.032.29015723
TTTGGGA173600.032.1052745