Basic Statistics
Measure | Value |
---|---|
Filename | H2HY7BGXC_n01_S20.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 8296923 |
Sequences flagged as poor quality | 0 |
Sequence length | 50 |
%GC | 41 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
TCAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGC | 43676 | 0.5264120204562582 | No Hit |
GCCAATAGGCAGCTTTCTTAACTATCCTAACAAGCCTTGGACCAAATGGA | 26223 | 0.31605692857460527 | No Hit |
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG | 21568 | 0.25995179176665856 | No Hit |
CAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGCG | 20117 | 0.2424633807014962 | No Hit |
GCAGGAAGGGGGGTCGCCGTGGTCGCCGTCTGTGAACAAGATTCCTCAAA | 16844 | 0.20301502135189153 | No Hit |
CTGGACCTGCGGCCTAAGAAGACACGTGCCATGCGCCGCCGGCTCAACAA | 12675 | 0.15276747777459185 | No Hit |
CTCTTATACACATCTCCGAGCCCACGAGACACGTTCGAATCTCGTATGCC | 10149 | 0.12232245616838916 | No Hit |
GCAAAGAGGAGGCCATGCGCTGGTTCCAGCAGAAGTATGATGGGATCATC | 9592 | 0.11560912400898503 | No Hit |
GCTGGATTGCAGAGTTAAGTTTATGATTATGAAATAAAAACTAAATAACA | 9240 | 0.11136658734810484 | No Hit |
GCTGGAGCTGGCAAGGTCACCAAGTCTGCCCAGAAAGCTCAGAAGGCTAA | 8737 | 0.1053040988809948 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGCAACG | 110 | 0.0 | 42.00135 | 20 |
ATACCGC | 1075 | 0.0 | 38.882866 | 35 |
TATACCG | 1105 | 0.0 | 38.02631 | 34 |
CGCCGTC | 2375 | 0.0 | 37.146458 | 24 |
CTACTCG | 3225 | 0.0 | 36.98152 | 2 |
CGGAACT | 1310 | 0.0 | 36.78646 | 2 |
TACTCGG | 3265 | 0.0 | 36.663246 | 3 |
ACGTGCC | 2135 | 0.0 | 36.581974 | 24 |
CGCATAC | 540 | 0.0 | 36.25846 | 35 |
CGTAACC | 780 | 0.0 | 35.817997 | 42 |
CACGTGC | 2190 | 0.0 | 35.462326 | 23 |
CACGGGC | 1460 | 0.0 | 35.418503 | 4 |
CGCCGTG | 2520 | 0.0 | 35.35466 | 15 |
ATGCGCC | 2085 | 0.0 | 35.23973 | 31 |
GCTACTC | 6700 | 0.0 | 35.043495 | 1 |
ACCTGCG | 2310 | 0.0 | 34.960037 | 5 |
TCCTAAC | 4145 | 0.0 | 34.87205 | 25 |
GTCGAAT | 1365 | 0.0 | 34.650414 | 10 |
CGGCCTA | 2400 | 0.0 | 34.55674 | 10 |
CAATAGG | 4490 | 0.0 | 34.50178 | 3 |