FastQCFastQC Report
Wed 28 Aug 2019
H2HY7BGXC_n01_S20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HY7BGXC_n01_S20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8296923
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGC436760.5264120204562582No Hit
GCCAATAGGCAGCTTTCTTAACTATCCTAACAAGCCTTGGACCAAATGGA262230.31605692857460527No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG215680.25995179176665856No Hit
CAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGCG201170.2424633807014962No Hit
GCAGGAAGGGGGGTCGCCGTGGTCGCCGTCTGTGAACAAGATTCCTCAAA168440.20301502135189153No Hit
CTGGACCTGCGGCCTAAGAAGACACGTGCCATGCGCCGCCGGCTCAACAA126750.15276747777459185No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACGTTCGAATCTCGTATGCC101490.12232245616838916No Hit
GCAAAGAGGAGGCCATGCGCTGGTTCCAGCAGAAGTATGATGGGATCATC95920.11560912400898503No Hit
GCTGGATTGCAGAGTTAAGTTTATGATTATGAAATAAAAACTAAATAACA92400.11136658734810484No Hit
GCTGGAGCTGGCAAGGTCACCAAGTCTGCCCAGAAAGCTCAGAAGGCTAA87370.1053040988809948No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAACG1100.042.0013520
ATACCGC10750.038.88286635
TATACCG11050.038.0263134
CGCCGTC23750.037.14645824
CTACTCG32250.036.981522
CGGAACT13100.036.786462
TACTCGG32650.036.6632463
ACGTGCC21350.036.58197424
CGCATAC5400.036.2584635
CGTAACC7800.035.81799742
CACGTGC21900.035.46232623
CACGGGC14600.035.4185034
CGCCGTG25200.035.3546615
ATGCGCC20850.035.2397331
GCTACTC67000.035.0434951
ACCTGCG23100.034.9600375
TCCTAAC41450.034.8720525
GTCGAAT13650.034.65041410
CGGCCTA24000.034.5567410
CAATAGG44900.034.501783