FastQCFastQC Report
Wed 28 Aug 2019
H2HY7BGXC_n01_S1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HY7BGXC_n01_S1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8389636
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTATACACATCTCCGAGCCCACGAGACCTCTGGTAATCTCGTATGCC352440.4200897392926225No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG244330.29122836795303153No Hit
CTGGACCTGCGGCCTAAGAAGACACGTGCCATGCGCCGCCGGCTCAACAA205310.24471860280946633No Hit
TCAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGC188830.22507531911992368No Hit
GCCAATAGGCAGCTTTCTTAACTATCCTAACAAGCCTTGGACCAAATGGA167800.200008677372892No Hit
TCTCTTATACACATCTCCGAGCCCACGAGACCTCTGGTAATCTCGTATGC140840.16787379094873725No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTCTGGTACTCTCGTATGCC129600.1544763086265006No Hit
GCCTTCGGAGCGTTCTCTGTCCTACTTCTGACTTTACTTGTGGTGTGACC102710.12242485848015336No Hit
AGCAATATCAACCATTAACCTTCCCTCTACACTTATCATCTTCACAATTC92650.11043387341238642No Hit
CCCTCTACACTTATCATCTTCACAATTCTAATTCTACTGACTATCCTAGA90500.10787118773686963No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTCT15850.038.16898310
CGGCCTA31000.037.8954210
GACACGT29600.037.75878521
GAGCGTT16300.036.8453068
AGACACG30900.036.52623420
TCCGACG13050.036.242441
ACGTGCC30900.035.9564124
GTATGCC83200.035.8001144
GCGACGT7450.035.73005313
ACACGTG31100.035.72538822
TATACCG9450.035.61841234
ATACCGC9500.035.43094635
ATGCGCC30600.035.3698131
GTCGAAT11200.035.35587710
TCGGAGC17200.035.309065
CGGAGCG17550.035.231796
CTACTCG75100.034.808242
CGACGGC13600.034.61496443
TACTCGG76000.034.5114363
CACGTGC32150.034.49018523