FastQCFastQC Report
Wed 28 Aug 2019
H2HY7BGXC_n01_S19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HY7BGXC_n01_S19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8132358
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTATACACATCTCCGAGCCCACGAGACCAGAACTGATCTCGTATGCC385920.4745487102264805No Hit
TCAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGC339940.4180091432275854No Hit
TCTCTTATACACATCTCCGAGCCCACGAGACCAGAACTGATCTCGTATGC187290.23030220755161046No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG183480.22561721950755242No Hit
GCCAATAGGCAGCTTTCTTAACTATCCTAACAAGCCTTGGACCAAATGGA160820.1977532223741257No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAGAACTGCTCTCGTATGCC140990.17336915074324077No Hit
CAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGCG137790.16943425264849382No Hit
GCAGGAAGGGGGGTCGCCGTGGTCGCCGTCTGTGAACAAGATTCCTCAAA100890.12405995899344323No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC87650.038.5266244
ATACCGC8100.036.394235
TATACCG8050.036.3469734
CTACTCG26600.036.3129962
CACGGGC11950.036.273174
CGCAACG1250.035.20068420
TACTCGG27650.035.0936053
ACGGTGC4950.034.66520729
GTCGAAT9550.034.32358610
CGGTGCG5100.033.64564530
ACGGGCA13000.033.512675
GCTACTC57300.033.3692931
GAATCGC20950.033.18441825
CTCACGG13200.033.1709822
CGCATAC4300.032.74341235
TCACGGG13450.032.7184143
AGACACG10800.032.3895220
CGGGCAA13400.032.3480876
CGAATGA10300.032.03768512
CGCCGTC15550.031.974924