FastQCFastQC Report
Wed 28 Aug 2019
H2HY7BGXC_n01_S18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HY7BGXC_n01_S18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7665723
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCAATAGGCAGCTTTCTTAACTATCCTAACAAGCCTTGGACCAAATGGA423700.5527202065610771No Hit
TCAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGC325510.42463052734882284No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG202610.264306445719471No Hit
CAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGCG173880.2268279195582726No Hit
CTGGACCTGCGGCCTAAGAAGACACGTGCCATGCGCCGCCGGCTCAACAA157430.20536875647606886No Hit
GCCTTCGGAGCGTTCTCTGTCCTACTTCTGACTTTACTTGTGGTGTGACC132970.17346048115748508No Hit
GCAGGAAGGGGGGTCGCCGTGGTCGCCGTCTGTGAACAAGATTCCTCAAA116530.1520143631592219No Hit
GCAAAGAGGAGGCCATGCGCTGGTTCCAGCAGAAGTATGATGGGATCATC107860.1407042753827656No Hit
GCTGGAGCTGGCAAGGTCACCAAGTCTGCCCAGAAAGCTCAGAAGGCTAA101870.13289027010237653No Hit
GCCCTTGAGAAGGATTATGAGGAGGTTGGTGTGGATTCTGTTGAAGGAGA77370.10092981444803054No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAGCG19150.039.4110346
AGACACG25450.039.3339820
GAGCGTT19550.039.1607788
ATACCGC10150.038.79692535
TATACCG10350.038.2597834
TCCTAAC60850.038.1087225
TCGGAGC19850.038.0209735
AATAGGC62450.037.6639984
GACACGT26400.037.5849621
CGTTCTC20400.037.20506311
CGCCGGC24900.037.19573237
CAATAGG65150.037.1162763
CGGCCTA27750.037.0226110
ACACGTG26750.036.84669522
ACCTGCG27900.036.6723255
CGAATGA12750.036.23378812
AACTATC64700.036.14708720
ATGCGCC25750.035.88130631
CCAATAG66700.035.791462
ACGTGCC27500.035.7617824