FastQCFastQC Report
Wed 28 Aug 2019
H2HY7BGXC_n01_S17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HY7BGXC_n01_S17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7553238
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCAATAGGCAGCTTTCTTAACTATCCTAACAAGCCTTGGACCAAATGGA537190.7112049163550784No Hit
TCAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGC391190.5179103319662376No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG218400.2891475152775538No Hit
CTGGACCTGCGGCCTAAGAAGACACGTGCCATGCGCCGCCGGCTCAACAA213320.2824219228892298No Hit
CAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGCG187420.24813199319285317No Hit
GCAGGAAGGGGGGTCGCCGTGGTCGCCGTCTGTGAACAAGATTCCTCAAA184560.24434553763564715No Hit
GCAAAGAGGAGGCCATGCGCTGGTTCCAGCAGAAGTATGATGGGATCATC133660.17695722020145532No Hit
GCTGGAGCTGGCAAGGTCACCAAGTCTGCCCAGAAAGCTCAGAAGGCTAA122280.16189083410320182No Hit
GCCTTCGGAGCGTTCTCTGTCCTACTTCTGACTTTACTTGTGGTGTGACC110660.1465067034826653No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTCTACCAATCTCGTATGCC101920.13493550712952512No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACACG35500.039.91113720
CGGCCTA37650.039.26585410
AATAGGC78000.039.1838844
CGGAGCG17300.039.1753736
CAATAGG79950.039.0260623
ATGCGCC32700.038.88423531
ACGTGCC36000.038.806824
CGCCGCC30600.038.7510834
ATACCGC10850.038.7275635
CGCCGGC32850.038.5078237
TCCTAAC78750.038.4977425
GACACGT36900.038.21802521
GCGCCGC31200.038.21740333
ATAGGCA80700.038.0915345
CACGTGC36850.037.97136323
CGCAACG1450.037.9322520
CCAATAG82100.037.8432582
ACCTGCG38850.037.8347665
CGCCGTC29350.037.70478424
ACTCGAC3050.037.50939622