FastQCFastQC Report
Wed 28 Aug 2019
H2HY7BGXC_n01_S16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HY7BGXC_n01_S16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8459794
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGC310440.3669592900252654No Hit
GCCAATAGGCAGCTTTCTTAACTATCCTAACAAGCCTTGGACCAAATGGA294360.3479517349949656No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG221380.2616848589930204No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCTATCCGATCTCGTATGCC186270.22018266638643919No Hit
CAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGCG140830.16646977455952236No Hit
CTGGACCTGCGGCCTAAGAAGACACGTGCCATGCGCCGCCGGCTCAACAA116120.13726102550487637No Hit
GCTGGAGCTGGCAAGGTCACCAAGTCTGCCCAGAAAGCTCAGAAGGCTAA111920.13229636560890254No Hit
GCCTTCGGAGCGTTCTCTGTCCTACTTCTGACTTTACTTGTGGTGTGACC96010.11348976109820169No Hit
TCTCTTATACACATCTCCGAGCCCACGAGACTCTATCCGATCTCGTATGC92680.10955349503782243No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAC452.3554094E-839.10825731
AGACACG21200.036.84259820
TCGGAGC14650.036.346385
ACGTGCC21200.036.3236924
GCGTTCT14550.036.13567710
ATACCGC10000.036.07950235
CGGAGCG14650.036.0459986
CTACTCG59000.035.838912
TATACCG10100.035.7222834
GACACGT21750.035.40515521
TACTCGG59300.035.0637173
CGGCCTA23200.034.89483310
GAGCGTT15200.034.8800668
GTCGAAT11600.034.7051910
GCTACTC115950.034.6891751
CGTTCTC16100.033.88652811
ACACGTG22900.033.8193322
GTATGCC44750.033.77203844
ACACGTC12600.033.6965527
CACGTGC22850.033.60446523