FastQCFastQC Report
Wed 28 Aug 2019
H2HY7BGXC_n01_S15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HY7BGXC_n01_S15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8979579
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGC533470.5940924401912384No Hit
GCCAATAGGCAGCTTTCTTAACTATCCTAACAAGCCTTGGACCAAATGGA518400.5773099161998575No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCCTCAATATCTCGTATGCC364470.40588762568935577No Hit
CAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGCG246000.2739549370855805No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG232750.25919923417345064No Hit
TCTCTTATACACATCTCCGAGCCCACGAGACGCCTCAATATCTCGTATGC192370.2142305335250127No Hit
GCAGGAAGGGGGGTCGCCGTGGTCGCCGTCTGTGAACAAGATTCCTCAAA164310.1829818524899664No Hit
CTGGACCTGCGGCCTAAGAAGACACGTGCCATGCGCCGCCGGCTCAACAA135830.15126544351355448No Hit
GCCTGGGGCTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAA129490.1442049788748448No Hit
GCTGGATTGCAGAGTTAAGTTTATGATTATGAAATAAAAACTAAATAACA117540.13089700530503715No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCCTCAATCTCTCGTATGCC115610.12874768405066653No Hit
GCTGGAGCTGGCAAGGTCACCAAGTCTGCCCAGAAAGCTCAGAAGGCTAA114480.12748927316080186No Hit
GCAAAGAGGAGGCCATGCGCTGGTTCCAGCAGAAGTATGATGGGATCATC110820.12341335824318712No Hit
GCTCACGGGCAAGGATGTTAATTTTGAATTCCCAGAGTTTCAATTGTAAA109470.12190994700308333No Hit
CCCTGGGAGTGGGTGGAGGCAGCCAGGGCTTACCTGTACACTGACTTGAG102580.1142369814887758No Hit
GAGAATGAAGGAGGCTAAGGAGAAGCGCCAGGAACAAATTGCGAAGAGAC98160.10931470172488042No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAC5550.038.8459635
CTACTCG33800.037.82162
AGACACG22700.037.79880520
ATACCGC10750.037.65495335
GTATGCC76150.037.411144
ACGTGCC22800.037.15054724
TATACCG11000.036.99915334
CGCCGTC24350.036.3217224
TCGGAGC13950.036.277635
CACGGGC19250.036.233594
ACGGGCA19350.036.046545
CGGAGCG13750.036.005396
GCTACTC67600.035.9018251
ATGCGCC22500.035.88282431
GACACGT23700.035.832621
TACTCGG35800.035.831583
TCCTAAC77500.035.74072625
TGCCGTA12800.035.5773139
CGTTCTC14200.035.47766511
CTCACGG20000.035.4247132