FastQCFastQC Report
Wed 28 Aug 2019
H2HY7BGXC_n01_S14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HY7BGXC_n01_S14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7775055
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCAATAGGCAGCTTTCTTAACTATCCTAACAAGCCTTGGACCAAATGGA479190.6163171836083474No Hit
TCAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGC417680.5372052030500105No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTCCATCCATCTCGTATGCC332200.427263858583637No Hit
CAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGCG210400.27060901819987127No Hit
GCAGGAAGGGGGGTCGCCGTGGTCGCCGTCTGTGAACAAGATTCCTCAAA194850.25060915967797015No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG186340.23966389948366926No Hit
GCCTGGGGCTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAA180800.23253854795882473No Hit
TCTCTTATACACATCTCCGAGCCCACGAGACTTCCATCCATCTCGTATGC179680.2310980436794338No Hit
CTGGACCTGCGGCCTAAGAAGACACGTGCCATGCGCCGCCGGCTCAACAA133040.17111132975908208No Hit
GCAAAGAGGAGGCCATGCGCTGGTTCCAGCAGAAGTATGATGGGATCATC116540.14988961492876898No Hit
GCTGGATTGCAGAGTTAAGTTTATGATTATGAAATAAAAACTAAATAACA106720.13725947919339476No Hit
GAGAATGAAGGAGGCTAAGGAGAAGCGCCAGGAACAAATTGCGAAGAGAC86680.11148474190857814No Hit
GCTCACGGGCAAGGATGTTAATTTTGAATTCCCAGAGTTTCAATTGTAAA86270.11095741444915824No Hit
CCGGAACTGGTCGAATGAGGCACCTAAAAATTGTATACCGCAGATTCAGG81600.10495102606991205No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCGC11350.040.8973235
CGCAACG2200.040.00150320
ACGTGCC21500.039.39657224
AGACACG22100.039.22319420
GACACGT22100.038.82473821
CGCATAC3750.038.71871635
TATACCG11900.038.63737534
ATGCGCC20000.038.4977331
CGCCGTC29700.038.14933824
CGCCGCC18350.038.12407334
TACCGAT3950.037.87435525
GTATGCC57050.037.55782344
ACACGTG22850.037.55040422
CGGAGCG11400.037.4460226
CAATAGG75400.037.4122923
CGAATGA14600.037.3681412
CACGGGC15400.037.149274
ACCTGCG23950.037.0258945
TCCTAAC69700.036.96217325
GCGCCGC19150.036.87605733