FastQCFastQC Report
Wed 28 Aug 2019
H2HY7BGXC_n01_S12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HY7BGXC_n01_S12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8730021
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTATACACATCTCCGAGCCCACGAGACTTAGCCTCATCTCGTATGCC419260.48025084933930856No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG227030.26005664820279356No Hit
TCTCTTATACACATCTCCGAGCCCACGAGACTTAGCCTCATCTCGTATGC177840.2037108501800855No Hit
GCCCTTGAGAAGGATTATGAGGAGGTTGGTGTGGATTCTGTTGAAGGAGA146970.168350110498016No Hit
TCAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGC126020.14435245917506956No Hit
CTGGACCTGCGGCCTAAGAAGACACGTGCCATGCGCCGCCGGCTCAACAA110340.1263914485429073No Hit
ACAAAGGTGCTGCTTTTACAGGGAAGCTTATTCTGTTTTAAACATTGAAA105260.1205724476493241No Hit
CCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCA90570.10374545490784041No Hit
GCCTTCGGAGCGTTCTCTGTCCTACTTCTGACTTTACTTGTGGTGTGACC88880.10180960618536887No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCGC6850.038.21829235
GTATGCC67050.036.7789544
TCCGACG13250.035.36337741
GACACGT19900.034.60437421
CTACTCG71400.034.4220732
TATACCG7550.034.38348834
AGACACG20400.034.29546720
AGAATCG60050.034.29257624
ACGTGCC20450.034.10383624
CGCCGAT6850.034.04267
TACTCGG72350.034.0307163
GAATCGC58650.033.94828425
GCTACTC153650.033.7958951
CTACTTG84050.033.7178732
GAGCGTT14150.033.4273458
AATCGCT60450.033.41055326
GCTACTT87950.033.3737141
AGGCTGA337950.033.29147711
GCGTTCT14150.033.11639410
CGGCCTA22300.032.85180310