FastQCFastQC Report
Wed 28 Aug 2019
H2HY7BGXC_n01_S11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HY7BGXC_n01_S11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7408825
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG192580.2599332552732721No Hit
GCCCTTGAGAAGGATTATGAGGAGGTTGGTGTGGATTCTGTTGAAGGAGA125190.16897416256963824No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCAACCTAATCTCGTATGCC120700.16291382236724447No Hit
ACAAAGGTGCTGCTTTTACAGGGAAGCTTATTCTGTTTTAAACATTGAAA97840.13205872726107043No Hit
TCAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGC88080.11888524833559978No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTCG700.037.71189516
TCCGACG12550.035.93373541
GAATCGC74150.035.07196825
GTCGAAT6000.034.4649510
CTACTCG102750.034.3057332
AGAATCG78250.034.2744324
GCTACTC207350.034.233571
CGACGGC13000.034.18221743
AGGCTGA428000.034.04176311
TACTCGG103750.033.868583
GCACTTT146950.033.7501871
AATCGCT76700.033.73361226
CTACTTG106850.033.7101142
GCTACTT110150.033.579571
TCGCTTG86200.033.1031228
GACACGT12500.033.0901521
TTGGGAG282850.033.0687686
TACTTGG110200.032.944473
TTGAACC197200.032.93075632
GGAGGCT440000.032.703599
Produced by FastQC (version 0.11.5)